Campylobacter Jejuni Adenosine Triphosphate Phosphoribosyltransferase is an Active Hexamer That is Allosterically Controlled by the Twisting of a Regulatory Tail

Overview

Journal Protein Sci

Specialty Biochemistry

Date 2016 May 19

PMID 27191057

Citations 8

Authors

Gerd Mittelstadt

Gert-Jan Moggre

Santosh Panjikar

Ali Reza Nazmi

Emily J Parker

Affiliations

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Abstract

Adenosine triphosphate phosphoribosyltransferase (ATP-PRT) catalyzes the first committed step of the histidine biosynthesis in plants and microorganisms. Here, we present the functional and structural characterization of the ATP-PRT from the pathogenic ε-proteobacteria Campylobacter jejuni (CjeATP-PRT). This enzyme is a member of the long form (HisGL ) ATP-PRT and is allosterically inhibited by histidine, which binds to a remote regulatory domain, and competitively inhibited by AMP. In the crystalline form, CjeATP-PRT was found to adopt two distinctly different hexameric conformations, with an open homohexameric structure observed in the presence of substrate ATP, and a more compact closed form present when inhibitor histidine is bound. CjeATP-PRT was observed to adopt only a hexameric quaternary structure in solution, contradicting previous hypotheses favoring an allosteric mechanism driven by an oligomer equilibrium. Instead, this study supports the conclusion that the ATP-PRT long form hexamer is the active species; the tightening of this structure in response to remote histidine binding results in an inhibited enzyme.

Citing Articles

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