Effects of Ethanol Extract of Propolis on Histopathological Changes and Anti-oxidant Defense of Kidney in a Rat Model for Type 1 Diabetes Mellitus

Overview

Journal J Diabetes Investig

Specialty Endocrinology

Date 2016 May 17

PMID 27181714

Citations 28

Authors

Hamid Reza Sameni

Parisa Ramhormozi

Ahmad Reza Bandegi

Abbas Ali Taherian

Majid Mirmohammadkhani

Manouchehr Safari

Affiliations

Soon will be listed here.

Abstract

Aims/introduction: Oxidative stress has a key role in the pathogenesis of diabetes. Propolis and its constituents have a wide range of medicinal properties against oxidative stress. In the present study, we evaluated the anti-oxidant effects of ethanolic extracts of propolis on kidneys in diabetes mellitus rats.

Materials And Methods: A total of 40 male Wistar rats were randomly divided into the following five groups: control, diabetes mellitus, diabetes mellitus with vehicle treatment, diabetes mellitus with propolis treatment (100 mg/kg) and diabetes mellitus with propolis treatment (200 mg/kg). Diabetes mellitus in rats was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Diabetic groups were treated with vehicle or ethanolic extracts of Iranian propolis for 6 weeks. Serum concentration of malondialdehyde, superoxide dismutase and glutathione peroxidase were measured.

Results: The results showed that Iranian propolis significantly inhibited bodyweight loss in diabetes mellitus rats. The propolis extracts significantly reduced serum glucose levels and kidney weight in diabetes mellitus rats (P < 0.001). Furthermore, propolis extracts significantly reduced the malondialdehyde content, and increased the activity of superoxide dismutase and glutathione peroxidase (P < 0.001) along with the total anti-oxidant activity in the kidney tissue of diabetes mellitus rats. In the kidneys of the diabetes mellitus and vehicle group, the glomerular basement membrane thickness and glomerular area were significantly increased. Treatment of diabetes mellitus rats with the propolis extract significantly reduced the glomerular basement membrane thickness and glomerular area.

Conclusions: The present study results showed that the Iranian propolis extract could enhance the anti-oxidant levels and histopathological changes in the kidneys of rats. The final results showed that most of the favorable effects of propolis are mediated by a reduction of blood glucose levels in diabetic animals.

Citing Articles

Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root-Propolis Extract on HEK293T Cells.

Marcincakova D, Hudakova N, Milek M, Kolesarova M, Dzugan M, Cizkova D Pharmaceuticals (Basel). 2025; 18(1).

PMID: 39861090 PMC: 11769209. DOI: 10.3390/ph18010027.

An Examination of the Effects of Propolis and Quercetin in a Rat Model of Streptozotocin-Induced Diabetic Peripheral Neuropathy.

Turedi S, Celik H, Dagli S, Taskin S, Seker U, Deniz M Curr Issues Mol Biol. 2024; 46(3):1955-1974.

PMID: 38534744 PMC: 10969614. DOI: 10.3390/cimb46030128.

Propolis and its therapeutic effects on renal diseases: A review.

Salami F, Mohebbati R, Hosseinian S, Shahraki S, Hossienzadeh H, Khajavi Rad A Iran J Basic Med Sci. 2024; 27(4):383-390.

PMID: 38419887 PMC: 10897566. DOI: 10.22038/IJBMS.2024.73081.15880.

Exploring the Functional Properties of Propolis, Geopropolis, and Cerumen, with a Special Emphasis on Their Antimicrobial Effects.

Chuttong B, Lim K, Praphawilai P, Danmek K, Maitip J, Vit P Foods. 2023; 12(21).

PMID: 37959028 PMC: 10648409. DOI: 10.3390/foods12213909.

Propolis and its constituents against cardiovascular risk factors including obesity, hypertension, atherosclerosis, diabetes, and dyslipidemia: A comprehensive review.

Khoshandam A, Hedayatian A, Mollazadeh A, Razavi B, Hosseinzadeh H Iran J Basic Med Sci. 2023; 26(8):853-871.

PMID: 37427329 PMC: 10329244. DOI: 10.22038/IJBMS.2023.67793.14835.

References

Boutabet K, Kebsa W, Alyane M, Lahouel M . Polyphenolic fraction of Algerian propolis protects rat kidney against acute oxidative stress induced by doxorubicin. Indian J Nephrol. 2011; 21(2):101-6. PMC: 3132328. DOI: 10.4103/0971-4065.82131. View

Lee E, Lee M, Hong S, Chung C, Hong S . Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats. Yonsei Med J. 2007; 48(5):847-55. PMC: 2628153. DOI: 10.3349/ymj.2007.48.5.847. View

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

Chance B, GREENSTEIN D, ROUGHTON F . The mechanism of catalase action. I. Steady-state analysis. Arch Biochem Biophys. 1952; 37(2):301-21. DOI: 10.1016/0003-9861(52)90194-x. View

Al-Hariri M, Eldin T, Abu-Hozaifa B, Elnour A . Glycemic control and anti-osteopathic effect of propolis in diabetic rats. Diabetes Metab Syndr Obes. 2012; 4:377-84. PMC: 3257965. DOI: 10.2147/DMSO.S24159. View

Tao B, Pietropaolo M, Atkinson M, Schatz D, Taylor D . Estimating the cost of type 1 diabetes in the U.S.: a propensity score matching method. PLoS One. 2010; 5(7):e11501. PMC: 2901386. DOI: 10.1371/journal.pone.0011501. View

Lee E, Uhm K, Lee Y, Han M, Lee M, Park J . CAPE (caffeic acid phenethyl ester) stimulates glucose uptake through AMPK (AMP-activated protein kinase) activation in skeletal muscle cells. Biochem Biophys Res Commun. 2007; 361(4):854-8. DOI: 10.1016/j.bbrc.2007.07.068. View

. Retraction statement. Protective effects of quercetin on streptozotocin-induced diabetic nephropathy in rats. Phytother Res. 2013; 27(10):1580. DOI: 10.1002/ptr.4910. View

Fuliang H, Hepburn H, Xuan H, Chen M, Daya S, Radloff S . Effects of propolis on blood glucose, blood lipid and free radicals in rats with diabetes mellitus. Pharmacol Res. 2005; 51(2):147-52. DOI: 10.1016/j.phrs.2004.06.011. View

10.

Sottile J . Regulation of angiogenesis by extracellular matrix. Biochim Biophys Acta. 2004; 1654(1):13-22. DOI: 10.1016/j.bbcan.2003.07.002. View

11.

Hansen J, Gundersen H . Renal hypertrophy in experimental diabetes. A morphometric study. Diabetologia. 1980; 18(6):501-5. DOI: 10.1007/BF00261707. View

12.

Yilmaz H, Uz E, Yucel N, Altuntas I, Ozcelik N . Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver. J Biochem Mol Toxicol. 2004; 18(4):234-8. DOI: 10.1002/jbt.20028. View

13.

Valavanidis A, Vlahogianni T, Dassenakis M, Scoullos M . Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicol Environ Saf. 2006; 64(2):178-89. DOI: 10.1016/j.ecoenv.2005.03.013. View

14.

Brownlee M . The pathobiology of diabetic complications: a unifying mechanism. Diabetes. 2005; 54(6):1615-25. DOI: 10.2337/diabetes.54.6.1615. View

15.

Mason R, Wahab N . Extracellular matrix metabolism in diabetic nephropathy. J Am Soc Nephrol. 2003; 14(5):1358-73. DOI: 10.1097/01.asn.0000065640.77499.d7. View

16.

Curtis S, Moritz M, SNODGRASS P . Serum enzymes derived from liver cell fractions. I. The response to carbon tetrachloride intoxication in rats. Gastroenterology. 1972; 62(1):84-92. View

17.

Sforcin J, Bankova V . Propolis: is there a potential for the development of new drugs?. J Ethnopharmacol. 2010; 133(2):253-60. DOI: 10.1016/j.jep.2010.10.032. View

18.

Montilla P, Barcos M, Munoz M, Bujalance I, Munoz-Castaneda J, Tunez I . Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats. J Biochem Mol Biol. 2005; 38(5):539-44. DOI: 10.5483/bmbrep.2005.38.5.539. View

19.

Li Y, Chen M, Xuan H, Hu F . Effects of encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus rats. Evid Based Complement Alternat Med. 2011; 2012:981896. PMC: 3118452. DOI: 10.1155/2012/981896. View

20.

Giugliano D, Ceriello A, Paolisso G . Oxidative stress and diabetic vascular complications. Diabetes Care. 1996; 19(3):257-67. DOI: 10.2337/diacare.19.3.257. View