Low Glucose Dependent Decrease of Apoptosis and Induction of Autophagy in Breast Cancer MCF-7 Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2016 May 11

PMID 27160935

Citations 20

Authors

Rafal Kretowski

Malgorzata Borzym-Kluczyk

Anna Stypulkowska

Justyna Branska-Januszewska

Halina Ostrowska

Marzanna Cechowska-Pasko

Affiliations

Soon will be listed here.

Abstract

Cancer cells have developed a number of adaptation mechanisms involving the signal activation of the transduction pathways, which promotes the progression and metastasis. Our results showed that the percentage of apoptotic MCF-7 cells incubated in the low glucose medium for 48 h was lower in comparison to those cultured in the high glucose medium, despite the high expression of the proapoptotic transcription factor-CHOP. Furthermore, the MCF-7 cells incubated in the low glucose medium for 48 h showed a higher expression of NF-κB p100/p52 subunits compared to cells incubated in the high glucose medium. Moreover, our findings demonstrated that the shortage of glucose strongly induces autophagy in MCF-7 cells. The activation of this process is not associated with the changes in the expression of mTOR kinase. We suggest, that the antiapoptotic chaperone ORP150 induction, transcription factor NF-κB2 activation, and increased autophagy constitute mechanisms protecting the MCF-7 cells against apoptosis.

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