Dynamic QTLs for Sugars and Enzyme Activities Provide an Overview of Genetic Control of Sugar Metabolism During Peach Fruit Development

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2016 Apr 28

PMID 27117339

Citations 28

Authors

Elsa Desnoues

Valentina Baldazzi

Michel Genard

Jehan-Baptiste Mauroux

Patrick Lambert

Carole Confolent

Benedicte Quilot-Turion

Affiliations

Soon will be listed here.

Abstract

Knowledge of the genetic control of sugar metabolism is essential to enhance fruit quality and promote fruit consumption. The sugar content and composition of fruits varies with species, cultivar and stage of development, and is controlled by multiple enzymes. A QTL (quantitative trait locus) study was performed on peach fruit [Prunus persica (L.) Batsch], the model species for Prunus Progeny derived from an interspecific cross between P. persica cultivars and P. davidiana was used. Dynamic QTLs for fresh weight, sugars, acids, and enzyme activities related to sugar metabolism were detected at different stages during fruit development. Changing effects of alleles during fruit growth were observed, including inversions close to maturity. This QTL analysis was supplemented by the identification of genes annotated on the peach genome as enzymes linked to sugar metabolism or sugar transporters. Several cases of co-locations between annotated genes, QTLs for enzyme activities and QTLs controlling metabolite concentrations were observed and discussed. These co-locations raise hypotheses regarding the functional regulation of sugar metabolism and pave the way for further analyses to enable the identification of the underlying genes. In conclusion, we identified the potential impact on fruit breeding of the modification of QTL effect close to maturity.

Citing Articles

gene integration into regulatory networks via interaction with conserved genes in peach.

Cao Y, Hong J, Zhao Y, Li X, Feng X, Wang H Hortic Res. 2024; 11(12):uhae252.

PMID: 39664695 PMC: 11630308. DOI: 10.1093/hr/uhae252.

Multiple Localization Analysis of the Major QTL- for Controlling Single Fruit Weight Traits in Melon Based on SLAF Sequencing.

Cai Y, Wang D, Che Y, Wang L, Zhang F, Liu T Genes (Basel). 2024; 15(9).

PMID: 39336729 PMC: 11430989. DOI: 10.3390/genes15091138.

Transcriptome and weighted gene co-expression network analyses reveal key genes and pathways involved in early fruit ripening in Citrus sinensis.

Chen J, Xie L, Lin Y, Zhong B, Wan S BMC Genomics. 2024; 25(1):735.

PMID: 39080567 PMC: 11289947. DOI: 10.1186/s12864-024-10651-1.

An overview of fermentation in the food industry - looking back from a new perspective.

Siddiqui S, Erol Z, Rugji J, Tasci F, Kahraman H, Toppi V Bioresour Bioprocess. 2024; 10(1):85.

PMID: 38647968 PMC: 10991178. DOI: 10.1186/s40643-023-00702-y.

Detection of two homologous major QTLs and development of diagnostic molecular markers for sucrose content in peanut.

Wang Z, Zhang Y, Huai D, Chen Y, Wang X, Kang Y Theor Appl Genet. 2024; 137(3):61.

PMID: 38411751 DOI: 10.1007/s00122-024-04549-5.

References

Desnoues E, Gibon Y, Baldazzi V, Signoret V, Genard M, Quilot-Turion B . Profiling sugar metabolism during fruit development in a peach progeny with different fructose-to-glucose ratios. BMC Plant Biol. 2014; 14:336. PMC: 4247632. DOI: 10.1186/s12870-014-0336-x. View

Han Y, Xie D, Teng W, Zhang S, Chang W, Li W . Dynamic QTL analysis of linolenic acid content in different developmental stages of soybean seed. Theor Appl Genet. 2011; 122(8):1481-8. DOI: 10.1007/s00122-011-1547-2. View

Moellering H, Gruber W . Determination of citrate with citrate lyase. Anal Biochem. 1966; 17(3):369-76. DOI: 10.1016/0003-2697(66)90172-2. View

Ogundiwin E, Peace C, Gradziel T, Parfitt D, Bliss F, Crisosto C . A fruit quality gene map of Prunus. BMC Genomics. 2009; 10:587. PMC: 2797820. DOI: 10.1186/1471-2164-10-587. View

Verde I, Bassil N, Scalabrin S, Gilmore B, Lawley C, Gasic K . Development and evaluation of a 9K SNP array for peach by internationally coordinated SNP detection and validation in breeding germplasm. PLoS One. 2012; 7(4):e35668. PMC: 3334984. DOI: 10.1371/journal.pone.0035668. View

Costa F, Peace C, Stella S, Serra S, Musacchi S, Bazzani M . QTL dynamics for fruit firmness and softening around an ethylene-dependent polygalacturonase gene in apple (Malus x domestica Borkh.). J Exp Bot. 2010; 61(11):3029-39. PMC: 2892147. DOI: 10.1093/jxb/erq130. View

Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L . Candidate genes and QTLs for sugar and organic acid content in peach [ Prunus persica (L.) Batsch]. Theor Appl Genet. 2003; 105(1):145-159. DOI: 10.1007/s00122-001-0841-9. View

Arcade A, Labourdette A, Falque M, Mangin B, Chardon F, Charcosset A . BioMercator: integrating genetic maps and QTL towards discovery of candidate genes. Bioinformatics. 2004; 20(14):2324-6. DOI: 10.1093/bioinformatics/bth230. View

Biais B, Benard C, Beauvoit B, Colombie S, Prodhomme D, Menard G . Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism. Plant Physiol. 2014; 164(3):1204-21. PMC: 3938614. DOI: 10.1104/pp.113.231241. View

10.

Sun Y, Liang Y, Wu J, Li Y, Cui X, Qin L . Dynamic QTL analysis for fruit lycopene content and total soluble solid content in a Solanum lycopersicum x S. pimpinellifolium cross. Genet Mol Res. 2012; 11(4):3696-710. DOI: 10.4238/2012.August.17.8. View

11.

Gibon Y, Pyl E, Sulpice R, Lunn J, Hohne M, Gunther M . Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environ. 2009; 32(7):859-74. DOI: 10.1111/j.1365-3040.2009.01965.x. View

12.

Kruglyak L, Lander E . A nonparametric approach for mapping quantitative trait loci. Genetics. 1995; 139(3):1421-8. PMC: 1206467. DOI: 10.1093/genetics/139.3.1421. View

13.

Shulaev V, Korban S, Sosinski B, Abbott A, Aldwinckle H, Folta K . Multiple models for Rosaceae genomics. Plant Physiol. 2008; 147(3):985-1003. PMC: 2442536. DOI: 10.1104/pp.107.115618. View

14.

Thevenot C, Simond-Cote E, Reyss A, Manicacci D, Trouverie J, Le Guilloux M . QTLs for enzyme activities and soluble carbohydrates involved in starch accumulation during grain filling in maize. J Exp Bot. 2005; 56(413):945-58. DOI: 10.1093/jxb/eri087. View

15.

Cirilli M, Bassi D, Ciacciulli A . Sugars in peach fruit: a breeding perspective. Hortic Res. 2016; 3:15067. PMC: 4720000. DOI: 10.1038/hortres.2015.67. View

16.

Lerceteau-Kohler E, Moing A, Guerin G, Renaud C, Petit A, Rothan C . Genetic dissection of fruit quality traits in the octoploid cultivated strawberry highlights the role of homoeo-QTL in their control. Theor Appl Genet. 2012; 124(6):1059-77. PMC: 3304055. DOI: 10.1007/s00122-011-1769-3. View

17.

Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J . A NAC Gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science. 2006; 314(5803):1298-301. PMC: 4737439. DOI: 10.1126/science.1133649. View

18.

van Ooijen J . Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species. Genet Res (Camb). 2011; 93(5):343-9. DOI: 10.1017/S0016672311000279. View

19.

Soller M, Brody T, Genizi A . On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor Appl Genet. 2014; 47(1):35-9. DOI: 10.1007/BF00277402. View

20.

Keurentjes J, Sulpice R, Gibon Y, Steinhauser M, Fu J, Koornneef M . Integrative analyses of genetic variation in enzyme activities of primary carbohydrate metabolism reveal distinct modes of regulation in Arabidopsis thaliana. Genome Biol. 2008; 9(8):R129. PMC: 2575519. DOI: 10.1186/gb-2008-9-8-r129. View