Functional Analysis Reveals the Regulatory Role of Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Feb 13

PMID 32046163

Citations 16

Authors

Qian Peng

Lu Wang

Collins Ogutu

Jingjing Liu

Li Liu

Md Dulal Ali Mollah

Yuepeng Han

Affiliations

Soon will be listed here.

Abstract

Sugar content is related to fruit sweetness, and the complex mechanisms underlying fruit sugar accumulation still remain elusive. Here, we report a peach gene encoding tonoplast sugar transporter that is located in the quantitative trait loci (QTL) interval on Chr5 controlling fruit sucrose content. One derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker was developed based on a nonsynonymous G/T variant in the third exon of . Genotyping of peach cultivars with the dCAPS marker revealed a significant difference in fruit sugar content among genotypes. is located in the tonoplast, and substitution of glutamine by histidine caused by the G/T variation has no impact on subcellular location. The expression profile of exhibited a consistency with the sugar accumulation pattern, and its transient silencing significantly inhibited sugar accumulation in peach fruits. All of these results demonstrated the role of in regulating sugar accumulation in peach fruit. In addition, cis-elements for binding of MYB and WRKY transcript factors were found in the promoter sequence of , suggesting a gene regulatory network of fruit sugar accumulation. Our results are not only helpful for understanding the mechanisms underlying fruit sugar accumulation, but will also be useful for the genetic improvement of fruit sweetness in peach breeding programs.

Citing Articles

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References

Nelson B, Cai X, Nebenfuhr A . A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J. 2007; 51(6):1126-36. DOI: 10.1111/j.1365-313X.2007.03212.x. View

Kelley L, Mezulis S, Yates C, Wass M, Sternberg M . The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc. 2015; 10(6):845-58. PMC: 5298202. DOI: 10.1038/nprot.2015.053. View

Diallinas G . Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters. Front Pharmacol. 2014; 5:207. PMC: 4162363. DOI: 10.3389/fphar.2014.00207. View

Zhen Q, Fang T, Peng Q, Liao L, Zhao L, Owiti A . Developing gene-tagged molecular markers for evaluation of genetic association of apple genes with fruit sugar accumulation. Hortic Res. 2018; 5:14. PMC: 5859117. DOI: 10.1038/s41438-018-0024-3. View

Hernandez Mora J, Micheletti D, Bink M, van de Weg E, Cantin C, Nazzicari N . Integrated QTL detection for key breeding traits in multiple peach progenies. BMC Genomics. 2017; 18(1):404. PMC: 5460339. DOI: 10.1186/s12864-017-3783-6. View

Kim C, Lee C, Shin J, Chung Y, Hyung N . A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP. Nucleic Acids Res. 1997; 25(5):1085-6. PMC: 146538. DOI: 10.1093/nar/25.5.1085. View

Vimolmangkang S, Zheng H, Peng Q, Jiang Q, Wang H, Fang T . Assessment of Sugar Components and Genes Involved in the Regulation of Sucrose Accumulation in Peach Fruit. J Agric Food Chem. 2016; 64(35):6723-9. DOI: 10.1021/acs.jafc.6b02159. View

Schulz A, Beyhl D, Marten I, Wormit A, Neuhaus E, Poschet G . Proton-driven sucrose symport and antiport are provided by the vacuolar transporters SUC4 and TMT1/2. Plant J. 2011; 68(1):129-36. DOI: 10.1111/j.1365-313X.2011.04672.x. View

Font I Forcada C, Guajardo V, Chin-Wo S, Moreno M . Association Mapping Analysis for Fruit Quality Traits in Using SNP Markers. Front Plant Sci. 2019; 9:2005. PMC: 6344403. DOI: 10.3389/fpls.2018.02005. View

10.

Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L . Candidate genes and QTLs for sugar and organic acid content in peach [ Prunus persica (L.) Batsch]. Theor Appl Genet. 2003; 105(1):145-159. DOI: 10.1007/s00122-001-0841-9. View

11.

Chen L, Cheung L, Feng L, Tanner W, Frommer W . Transport of sugars. Annu Rev Biochem. 2015; 84:865-94. DOI: 10.1146/annurev-biochem-060614-033904. View

12.

Wang L, Zhao S, Gu C, Zhou Y, Zhou H, Ma J . Deep RNA-Seq uncovers the peach transcriptome landscape. Plant Mol Biol. 2013; 83(4-5):365-77. DOI: 10.1007/s11103-013-0093-5. View

13.

Afoufa-Bastien D, Medici A, Jeauffre J, Coutos-Thevenot P, Lemoine R, Atanassova R . The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling. BMC Plant Biol. 2010; 10:245. PMC: 3095327. DOI: 10.1186/1471-2229-10-245. View

14.

Verde I, Abbott A, Scalabrin S, Jung S, Shu S, Marroni F . The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat Genet. 2013; 45(5):487-94. DOI: 10.1038/ng.2586. View

15.

Cho J, Burla B, Lee D, Ryoo N, Hong S, Kim H . Expression analysis and functional characterization of the monosaccharide transporters, OsTMTs, involving vacuolar sugar transport in rice (Oryza sativa). New Phytol. 2010; 186(3):657-68. DOI: 10.1111/j.1469-8137.2010.03194.x. View

16.

Wei X, Liu F, Chen C, Ma F, Li M . The Malus domestica sugar transporter gene family: identifications based on genome and expression profiling related to the accumulation of fruit sugars. Front Plant Sci. 2014; 5:569. PMC: 4220645. DOI: 10.3389/fpls.2014.00569. View

17.

Tong Z, Gao Z, Wang F, Zhou J, Zhang Z . Selection of reliable reference genes for gene expression studies in peach using real-time PCR. BMC Mol Biol. 2009; 10:71. PMC: 3224724. DOI: 10.1186/1471-2199-10-71. View

18.

Dirlewanger E, Graziano E, Joobeur T, Garriga-Caldere F, Cosson P, Howad W . Comparative mapping and marker-assisted selection in Rosaceae fruit crops. Proc Natl Acad Sci U S A. 2004; 101(26):9891-6. PMC: 470769. DOI: 10.1073/pnas.0307937101. View

19.

Jung B, Ludewig F, Schulz A, Meissner G, Wostefeld N, Flugge U . Identification of the transporter responsible for sucrose accumulation in sugar beet taproots. Nat Plants. 2016; 1:14001. DOI: 10.1038/nplants.2014.1. View

20.

Han L, Zhu Y, Liu M, Zhou Y, Lu G, Lan L . Molecular mechanism of substrate recognition and transport by the AtSWEET13 sugar transporter. Proc Natl Acad Sci U S A. 2017; 114(38):10089-10094. PMC: 5617298. DOI: 10.1073/pnas.1709241114. View