Do Nucleic Acids Moonlight As Molecular Chaperones?

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2016 Apr 24

PMID 27105849

Citations 35

Authors

Brianne E Docter

Scott Horowitz

Michael J Gray

Ursula Jakob

James C A Bardwell

Affiliations

Soon will be listed here.

Abstract

Organisms use molecular chaperones to combat the unfolding and aggregation of proteins. While protein chaperones have been widely studied, here we demonstrate that DNA and RNA exhibit potent chaperone activity in vitro Nucleic acids suppress the aggregation of classic chaperone substrates up to 300-fold more effectively than the protein chaperone GroEL. Additionally, RNA cooperates with the DnaK chaperone system to refold purified luciferase. Our findings reveal a possible new role for nucleic acids within the cell: that nucleic acids directly participate in maintaining proteostasis by preventing protein aggregation.

Citing Articles

In the Beginning: Let Hydration Be Coded in Proteins for Manifestation and Modulation by Salts and Adenosine Triphosphate.

Song J Int J Mol Sci. 2024; 25(23).

PMID: 39684527 PMC: 11641266. DOI: 10.3390/ijms252312817.

Roles of Nucleic Acids in Protein Folding, Aggregation, and Disease.

Litberg T, Horowitz S ACS Chem Biol. 2024; 19(4):809-823.

PMID: 38477936 PMC: 11149768. DOI: 10.1021/acschembio.3c00695.

RNA-dependent proteome solubility maintenance in lysates analysed by quantitative mass spectrometry: Proteomic characterization in terms of isoelectric point, structural disorder, functional hub, and chaperone network.

Park C, Han B, Choi Y, Jin Y, Kim K, Choi S RNA Biol. 2024; 21(1):1-18.

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Why are G-quadruplexes good at preventing protein aggregation?.

Litberg T, Sannapureddi R, Huang Z, Son A, Sathyamoorthy B, Horowitz S RNA Biol. 2023; 20(1):495-509.

PMID: 37493593 PMC: 10373610. DOI: 10.1080/15476286.2023.2228572.

G-quadruplexes rescuing protein folding.

Son A, Huizar Cabral V, Huang Z, Litberg T, Horowitz S Proc Natl Acad Sci U S A. 2023; 120(20):e2216308120.

PMID: 37155907 PMC: 10194009. DOI: 10.1073/pnas.2216308120.

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