Regulation of Human Immunodeficiency Virus Env Expression by the Rev Gene Product

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 May 1

PMID 2704072

Citations 180

Authors

M L Hammarskjold

J Heimer

B Hammarskjold

I Sangwan

L Albert

D Rekosh

Affiliations

Soon will be listed here.

Abstract

A single simian virus 40 late replacement vector which expresses both the rev and envelope (env) genes of human immunodeficiency virus was used to examine the mechanism underlying the dependence of env gene expression on the rev protein. When rev was deleted from the vector, no envelope protein expression could be detected in transfected cells, and the levels of cytoplasmic env mRNA were dramatically reduced. In contrast to this, the levels of env RNA in total cellular RNA preparations were similar with or without rev coexpression, and analysis of nuclear RNA showed that the levels of nuclear env RNA were increased in the absence of rev. These results suggest that rev functions to regulate nuclear export of env mRNA. It was possible to restore env expression from the vector lacking rev by supplying rev in trans, provided that a cis-acting sequence was also present. This sequence was mapped to a 854-base-pair region within the env open reading frame, and it was shown that the sequence could be moved but that it worked only in its original orientation.

Citing Articles

Rev-RRE activity modulates HIV-1 replication and latency reactivation: Implications for viral persistence and cure strategies.

Dzhivhuho G, Jackson P, Honeycutt E, da Silva Mesquita F, Huang J, Hammarskjold M bioRxiv. 2025; .

PMID: 39829859 PMC: 11741256. DOI: 10.1101/2025.01.06.631466.

Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site.

Tipo J, Gottipati K, Slaton M, Gonzalez-Gutierrez G, Choi K Nat Commun. 2024; 15(1):4198.

PMID: 38760344 PMC: 11101469. DOI: 10.1038/s41467-024-48162-y.

Measurement of HIV Rev-Rev Response Element Functional Activity.

Jackson P, Dzhivhuho G, Huang J, Hammarskjold M, Rekosh D Methods Mol Biol. 2024; 2807:175-194.

PMID: 38743229 DOI: 10.1007/978-1-0716-3862-0_13.

Rev-Rev Response Element Activity Selection Bias at the Human Immunodeficiency Virus Transmission Bottleneck.

Jackson P, Holsey J, Turse L, Hammarskjold M, Rekosh D Open Forum Infect Dis. 2023; 10(10):ofad486.

PMID: 37854107 PMC: 10580148. DOI: 10.1093/ofid/ofad486.

Packaging cells for lentiviral vectors generated using the cumate and coumermycin gene induction systems and nanowell single-cell cloning.

Broussau S, Lytvyn V, Simoneau M, Guilbault C, Leclerc M, Nazemi-Moghaddam N Mol Ther Methods Clin Dev. 2023; 29:40-57.

PMID: 36936448 PMC: 10018046. DOI: 10.1016/j.omtm.2023.02.013.

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