Hypoxia Induces Tumor-Derived Exosome SNHG16 to Mediate Nasopharyngeal Carcinoma Progression Through the MiR-23b-5p/MCM6 Pathway

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2023 Apr 29

PMID 37119503

Authors

Wei Hou

Lu Xu

Tao Su

Yunxiao Wu

Yujuan Liu

Yangao Wei

Affiliations

Soon will be listed here.

Abstract

This study aims to investigate the mechanism of tumor-derived exosomal (EVs) SNHG16 in promoting the progression of nasopharyngeal carcinoma (NPC). QRT-PCR was used to detect the expression of SNHG16, miR-23b-5p and MCM6 in NPC. MTT, flow cytometry and transwell were used to detect the effects of them on the proliferation, cycle, apoptosis and invasion ability of NPC. Transmission electron microscopy, Western blotting and BCA were used to verify the regulation of exosome secretion under different oxygen environments. Our results showed that hypoxia induces tumor-derived exosome SNHG16 to mediate NPC progression through the miR-23b-5p/MCM6 pathway.

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References

Bai R, Li Y, Jian L, Yang Y, Zhao L, Wei M . The hypoxia-driven crosstalk between tumor and tumor-associated macrophages: mechanisms and clinical treatment strategies. Mol Cancer. 2022; 21(1):177. PMC: 9454207. DOI: 10.1186/s12943-022-01645-2. View

Bister N, Pistono C, Huremagic B, Jolkkonen J, Giugno R, Malm T . Hypoxia and extracellular vesicles: A review on methods, vesicular cargo and functions. J Extracell Vesicles. 2020; 10(1):e12002. PMC: 7710128. DOI: 10.1002/jev2.12002. View

Cao B, Dai W, Ma S, Wang Q, Lan M, Luo H . An EV-Associated Gene Signature Correlates with Hypoxic Microenvironment and Predicts Recurrence in Lung Adenocarcinoma. Mol Ther Nucleic Acids. 2019; 17:879-890. PMC: 6727147. DOI: 10.1016/j.omtn.2019.07.021. View

Catapano F, Domingos J, Perry M, Ricotti V, Phillips L, Servais L . Downregulation of miRNA-29, -23 and -21 in urine of Duchenne muscular dystrophy patients. Epigenomics. 2018; 10(7):875-889. DOI: 10.2217/epi-2018-0022. View

Chen F, Huang C, Wu Q, Jiang L, Chen S, Chen L . Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers. J Cell Biochem. 2019; 121(3):2525-2533. DOI: 10.1002/jcb.29475. View

Choudhry H, Harris A . Advances in Hypoxia-Inducible Factor Biology. Cell Metab. 2017; 27(2):281-298. DOI: 10.1016/j.cmet.2017.10.005. View

Dai J, Su Y, Zhong S, Cong L, Liu B, Yang J . Exosomes: key players in cancer and potential therapeutic strategy. Signal Transduct Target Ther. 2020; 5(1):145. PMC: 7406508. DOI: 10.1038/s41392-020-00261-0. View

De Felice F, Marchetti C, Serpone M, Camarda A, Vischioni B, Ingargiola R . Upper-neck irradiation versus standard whole-neck irradiation in nasopharyngeal carcinoma: A systematic review and meta-analysis. Tumori. 2023; 109(6):529-536. DOI: 10.1177/03008916231154765. View

Gao W, Yu-Wai Chan J, Wong T . Differential expression of long noncoding RNA in primary and recurrent nasopharyngeal carcinoma. Biomed Res Int. 2014; 2014:404567. PMC: 4009106. DOI: 10.1155/2014/404567. View

10.

Ha J, Kim H, Yoon Y, Park S . A method of extracting disease-related microRNAs through the propagation algorithm using the environmental factor based global miRNA network. Biomed Mater Eng. 2015; 26 Suppl 1:S1763-72. DOI: 10.3233/BME-151477. View

11.

He G, Peng X, Wei S, Yang S, Li X, Huang M . Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications. Mol Cancer. 2022; 21(1):19. PMC: 8762953. DOI: 10.1186/s12943-021-01440-5. View

12.

He R, Wu P, Xiang X, Yang X, Liang H, Qiu X . Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification. Int J Mol Med. 2018; 41(5):2813-2831. PMC: 5846654. DOI: 10.3892/ijmm.2018.3513. View

13.

Hotton J, Agopiantz M, Leroux A, Charra-Brunaud C, Marie B, Busby-Venner H . Minichromosome maintenance complex component 6 (MCM6) expression correlates with histological grade and survival in endometrioid endometrial adenocarcinoma. Virchows Arch. 2017; 472(4):623-633. DOI: 10.1007/s00428-017-2278-9. View

14.

Hsu W, Tse K, Liang S, Chien Y, Su W, Yu K . Evaluation of human leukocyte antigen-A (HLA-A), other non-HLA markers on chromosome 6p21 and risk of nasopharyngeal carcinoma. PLoS One. 2012; 7(8):e42767. PMC: 3413673. DOI: 10.1371/journal.pone.0042767. View

15.

Jiang H, Zhao H, Zhang M, He Y, Li X, Xu Y . Hypoxia Induced Changes of Exosome Cargo and Subsequent Biological Effects. Front Immunol. 2022; 13:824188. PMC: 9013908. DOI: 10.3389/fimmu.2022.824188. View

16.

Kumar A, Deep G . Hypoxia in tumor microenvironment regulates exosome biogenesis: Molecular mechanisms and translational opportunities. Cancer Lett. 2020; 479:23-30. DOI: 10.1016/j.canlet.2020.03.017. View

17.

Li W, Duan X, Chen X, Zhan M, Peng H, Meng Y . Immunotherapeutic approaches in EBV-associated nasopharyngeal carcinoma. Front Immunol. 2023; 13:1079515. PMC: 9875085. DOI: 10.3389/fimmu.2022.1079515. View

18.

Li X, Li X . USP21 Promotes the Progression of Nasopharyngeal Carcinoma by Regulating FOXM1. Stem Cells Int. 2023; 2023:9196583. PMC: 9938788. DOI: 10.1155/2023/9196583. View

19.

Lian D, Amin B, Du D, Yan W . Enhanced expression of the long non-coding RNA SNHG16 contributes to gastric cancer progression and metastasis. Cancer Biomark. 2017; 21(1):151-160. DOI: 10.3233/CBM-170462. View

20.

Lin M, Zhang X, You R, Liu Y, Cai H, Liu L . Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance. Nat Commun. 2023; 14(1):610. PMC: 9899247. DOI: 10.1038/s41467-023-35995-2. View