Non-essential Contribution of LRRC8A to Volume Regulation

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2016 Feb 14

PMID 26873248

Citations 24

Authors

Lalida Sirianant

Podchanart Wanitchakool

Jiraporn Ousingsawat

Roberta Benedetto

Anna Zormpa

Ines Cabrita

Rainer Schreiber

Karl Kunzelmann

Affiliations

Soon will be listed here.

Abstract

Volume regulation is an essential property of any living cell and needs to be tightly controlled. While different types of K(+) channels have been found to participate in the regulation of cell volume, the newly identified volume-regulated anion channel (VRAC) LRRC8 has been claimed to be essential for volume regulation. In unbiased genome-wide small interfering RNA (siRNA) screens, two independent studies identified LRRC8A/Swell1 as an essential component of VRAC, thus being indispensable for cellular volume regulation. We reanalyzed the role of LRRC8A for VRAC and regulatory volume decrease (RVD) in several cell types and under various conditions. While the role of LRRC8A for VRAC and its contribution to RVD is confirmed, we find that it is not essential for swelling-activated anion currents or cellular volume regulation, or apoptotic cell shrinkage. The contribution of LRRC8A is variable and largely depending on the cell type.

Citing Articles

Trends in volume-regulated anion channel (VRAC) research: visualization and bibliometric analysis from 2014 to 2022.

Liu T, Li Y, Wang D, Stauber T, Zhao J Front Pharmacol. 2023; 14:1234885.

PMID: 37538172 PMC: 10394876. DOI: 10.3389/fphar.2023.1234885.

The Role of Chloride Channels in the Multidrug Resistance.

Wilczynski B, Dabrowska A, Saczko J, Kulbacka J Membranes (Basel). 2022; 12(1).

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LRRC8A is essential for volume-regulated anion channel in smooth muscle cells contributing to cerebrovascular remodeling during hypertension.

Li X, Lv X, Huang C, Sun L, Ma M, Liu C Cell Prolif. 2021; 54(12):e13146.

PMID: 34725866 PMC: 8666279. DOI: 10.1111/cpr.13146.

Ca Dependence of Volume-Regulated VRAC/LRRC8 and TMEM16A Cl Channels.

Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K Front Cell Dev Biol. 2020; 8:596879.

PMID: 33335902 PMC: 7736618. DOI: 10.3389/fcell.2020.596879.

Ions, the Movement of Water and the Apoptotic Volume Decrease.

Bortner C, Cidlowski J Front Cell Dev Biol. 2020; 8:611211.

PMID: 33324655 PMC: 7723978. DOI: 10.3389/fcell.2020.611211.

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