Quality of Histone Modification Antibodies Undermines Chromatin Biology Research

Overview

Journal F1000Res

Specialties Biomedical Engineering
Science

Date 2016 Feb 5

PMID 26834995

Citations 5

Authors

Goran Kungulovski

Albert Jeltsch

Affiliations

Soon will be listed here.

Abstract

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.

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