MinION Analysis and Reference Consortium: Phase 1 Data Release and Analysis

Overview

Journal F1000Res

Specialties Biomedical Engineering
Science

Date 2016 Feb 3

PMID 26834992

Citations 140

Authors

Camilla L C Ip

Matthew Loose

John R Tyson

Mariateresa de Cesare

Bonnie L Brown

Miten Jain

Richard M Leggett

David A Eccles

Vadim Zalunin

John M Urban

Paolo Piazza

Rory J Bowden

Benedict Paten

Solomon Mwaigwisya

Elizabeth M Batty

Jared T Simpson

Terrance P Snutch

Ewan Birney

David Buck

Sara Goodwin

Hans J Jansen

Justin OGrady

Hugh E Olsen

Affiliations

Soon will be listed here.

Abstract

The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.

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