BPA-toxicity Via Superoxide Anion Overload and a Deficit in β-catenin Signaling in Human Bone Mesenchymal Stem Cells

Overview

Journal Environ Toxicol

Specialties Environmental Health
Toxicology

Date 2016 Jan 30

PMID 26822619

Citations 10

Authors

Yea-Hyun Leem

Seikwan Oh

Hong-Je Kang

Jung-Hwa Kim

Juno Yoon

Jae-Suk Chang

Affiliations

Soon will be listed here.

Abstract

Bisphenol A (BPA), used in the manufacture of products based on polycarbonate plastics and epoxy resins, is well known as an endocrine-disrupting monomer. In the current study, BPA increased cytotoxicity in hBMSCs in a dose- and time-dependent manner, concomitantly with increased lipid peroxidation. Increased cell death in BPA-treated cells was markedly blocked by pretreatment with the superoxide dismutase mimetic MnTBAP and MnTMPyP, but not by catalase, glutathione, the glutathione peroxidase mimetic ebselen, the NOS inhibitor NAME, or the xanthine oxidase inhibitor allopurinol. Furthermore, the decline in nuclear β-catenin and cyclin D1 levels in hBMSCs exposed to BPA was reversed by MnTBAP treatment. Finally, treatment of hBMSCs with the GSK3β inhibitor LiCl increased nuclear β-catenin levels and significantly attenuated cytotoxicity compared with BPA treatment. Our current results in hBMSCs exposed to BPA suggest that BPA causes a disturbance in β-catenin signaling via a superoxide anion overload. © 2016 The Authors Environmental Toxicology Published by Wiley Periodicals, Inc. Environ Toxicol 32: 344-352, 2017.

Citing Articles

Leaf Extract Protects C2C12 Mouse Myoblasts Against the Suppressive Effects of Bisphenol-A on Myogenic Differentiation.

Pitchakarn P, Karinchai J, Buacheen P, Imsumran A, Wongnoppavich A, Boonyapranai K Int J Mol Sci. 2025; 26(2).

PMID: 39859191 PMC: 11765284. DOI: 10.3390/ijms26020476.

Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-B Signaling Pathway.

Tipbunjong C, Thitiphatphuvanon T, Pholpramool C, Surinlert P J Toxicol. 2024; 2024:3840950.

PMID: 38449520 PMC: 10917485. DOI: 10.1155/2024/3840950.

Current Evidence on Bisphenol A Exposure and the Molecular Mechanism Involved in Related Pathological Conditions.

Della Rocca Y, Traini E, Diomede F, Fonticoli L, Trubiani O, Paganelli A Pharmaceutics. 2023; 15(3).

PMID: 36986769 PMC: 10053246. DOI: 10.3390/pharmaceutics15030908.

Morphological, immunohistochemical, and biochemical study on the ameliorative effect of gallic acid against bisphenol A-induced nephrotoxicity in male albino rats.

Saleh S, Mahmoud A, Al-Salahy M, Mohamed Moustafa F Sci Rep. 2023; 13(1):1732.

PMID: 36720896 PMC: 9889795. DOI: 10.1038/s41598-023-28860-1.

Bisphenol A induced toxicity in blood cells of freshwater fish after acute exposure.

Sharma P, Chadha P Saudi J Biol Sci. 2021; 28(8):4738-4750.

PMID: 34354462 PMC: 8324972. DOI: 10.1016/j.sjbs.2021.04.088.

References

Barnham K, Masters C, Bush A . Neurodegenerative diseases and oxidative stress. Nat Rev Drug Discov. 2004; 3(3):205-14. DOI: 10.1038/nrd1330. View

Sies H . Strategies of antioxidant defense. Eur J Biochem. 1993; 215(2):213-9. DOI: 10.1111/j.1432-1033.1993.tb18025.x. View

Sohoni P, Sumpter J . Several environmental oestrogens are also anti-androgens. J Endocrinol. 1998; 158(3):327-39. DOI: 10.1677/joe.0.1580327. View

Hassan Z, Elobeid M, Virk P, Omer S, Elamin M, Daghestani M . Bisphenol A induces hepatotoxicity through oxidative stress in rat model. Oxid Med Cell Longev. 2012; 2012:194829. PMC: 3409570. DOI: 10.1155/2012/194829. View

Chen D, Li M, Luo J, Gu W . Direct interactions between HIF-1 alpha and Mdm2 modulate p53 function. J Biol Chem. 2003; 278(16):13595-8. DOI: 10.1074/jbc.C200694200. View

Lahnsteiner F, Berger B, Kletzl M, Weismann T . Effect of bisphenol A on maturation and quality of semen and eggs in the brown trout, Salmo trutta f. fario. Aquat Toxicol. 2005; 75(3):213-24. DOI: 10.1016/j.aquatox.2005.08.004. View

Calafat A, Ye X, Wong L, Reidy J, Needham L . Exposure of the U.S. population to bisphenol A and 4-tertiary-octylphenol: 2003-2004. Environ Health Perspect. 2008; 116(1):39-44. PMC: 2199288. DOI: 10.1289/ehp.10753. View

Richter C, Birnbaum L, Farabollini F, Newbold R, Rubin B, Talsness C . In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol. 2007; 24(2):199-224. PMC: 2151845. DOI: 10.1016/j.reprotox.2007.06.004. View

Moriyama K, Tagami T, Akamizu T, Usui T, Saijo M, Kanamoto N . Thyroid hormone action is disrupted by bisphenol A as an antagonist. J Clin Endocrinol Metab. 2002; 87(11):5185-90. DOI: 10.1210/jc.2002-020209. View

10.

Bedard K, Krause K . The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev. 2007; 87(1):245-313. DOI: 10.1152/physrev.00044.2005. View

11.

Wetherill Y, Akingbemi B, Kanno J, McLachlan J, Nadal A, Sonnenschein C . In vitro molecular mechanisms of bisphenol A action. Reprod Toxicol. 2007; 24(2):178-98. DOI: 10.1016/j.reprotox.2007.05.010. View

12.

Kawamoto E, Gleichmann M, Yshii L, de Sa Lima L, Mattson M, Scavone C . Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults. Braz J Med Biol Res. 2011; 45(1):58-67. PMC: 3854138. DOI: 10.1590/s0100-879x2011007500157. View

13.

Hong Y, Park E, Park M, Ko J, Oh S, Kim H . Community level exposure to chemicals and oxidative stress in adult population. Toxicol Lett. 2008; 184(2):139-44. DOI: 10.1016/j.toxlet.2008.11.001. View

14.

Oh P, Lim K . Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation in bisphenol A-stimulated Chang liver cells. J Appl Toxicol. 2008; 28(6):749-58. DOI: 10.1002/jat.1334. View

15.

Bonefeld-Jorgensen E, Long M, Hofmeister M, Vinggaard A . Endocrine-disrupting potential of bisphenol A, bisphenol A dimethacrylate, 4-n-nonylphenol, and 4-n-octylphenol in vitro: new data and a brief review. Environ Health Perspect. 2008; 115 Suppl 1:69-76. PMC: 2174402. DOI: 10.1289/ehp.9368. View

16.

Ho Y, Magnenat J, Gargano M, Cao J . The nature of antioxidant defense mechanisms: a lesson from transgenic studies. Environ Health Perspect. 1998; 106 Suppl 5:1219-28. PMC: 1533365. DOI: 10.1289/ehp.98106s51219. View

17.

Yamamoto T, Yasuhara A . Quantities of bisphenol a leached from plastic waste samples. Chemosphere. 1999; 38(11):2569-76. DOI: 10.1016/s0045-6535(98)00464-0. View

18.

Lin C, Wang J, Ko J, Surendran K, Huang Y, Kuo Y . Superoxide destabilization of beta-catenin augments apoptosis of high-glucose-stressed mesangial cells. Endocrinology. 2008; 149(6):2934-42. DOI: 10.1210/en.2007-1372. View

19.

Kabuto H, Hasuike S, Minagawa N, Shishibori T . Effects of bisphenol A on the metabolisms of active oxygen species in mouse tissues. Environ Res. 2003; 93(1):31-5. DOI: 10.1016/s0013-9351(03)00062-8. View

20.

An W, Kanekal M, Simon M, Maltepe E, Blagosklonny M, Neckers L . Stabilization of wild-type p53 by hypoxia-inducible factor 1alpha. Nature. 1998; 392(6674):405-8. DOI: 10.1038/32925. View