Serial Femtosecond X-ray Diffraction of Enveloped Virus Microcrystals

Overview

Journal Struct Dyn

Date 2016 Jan 23

PMID 26798819

Citations 6

Authors

Robert M Lawrence

Chelsie E Conrad

Nadia A Zatsepin

Thomas D Grant

Haiguang Liu

Daniel James

Garrett Nelson

Ganesh Subramanian

Andrew Aquila

Mark S Hunter

Mengning Liang

Sebastien Boutet

Jesse Coe

John C H Spence

Uwe Weierstall

Wei Liu

Petra Fromme

Vadim Cherezov

Brenda G Hogue

Affiliations

Soon will be listed here.

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

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