Handheld Real-time PCR Device

Overview

Journal Lab Chip

Publisher Royal Society of Chemistry

Specialties Biotechnology
Chemistry

Date 2016 Jan 13

PMID 26753557

Citations 36

Authors

Christian D Ahrberg

Bojan Robert Ilic

Andreas Manz

Pavel Neuzil

Affiliations

Soon will be listed here.

Abstract

Here we report one of the smallest real-time polymerase chain reaction (PCR) systems to date with an approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in the form of virtual reaction chambers (VRCs) where a ≈200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate the PCR performance. The standard curve slope was -3.02 ± 0.16 cycles at threshold per decade (mean ± standard deviation) corresponding to an amplification efficiency of 0.91 ± 0.05 per cycle (mean ± standard deviation). The PCR device was capable of detecting a single deoxyribonucleic acid (DNA) copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.

Citing Articles

Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection.

Costantini F, Lovecchio N, Nandimandalam M, Manglli A, Faggioli F, Biasin M Biosensors (Basel). 2023; 13(5).

PMID: 37232905 PMC: 10216243. DOI: 10.3390/bios13050544.

Ultrafast Plasmonic Nucleic Acid Amplification and Real-Time Quantification for Decentralized Molecular Diagnostics.

Kang B, Jang K, Yu E, Na H, Lee Y, Ko W ACS Nano. 2023; 17(7):6507-6518.

PMID: 36971140 PMC: 10081571. DOI: 10.1021/acsnano.2c11831.

Aptamer against Aflatoxin B1 Obtained by SELEX and Applied in Detection.

Yang C, Tsai C Biosensors (Basel). 2022; 12(10).

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A Point-of-Care Device for Fully Automated, Fast and Sensitive Protein Quantification via qPCR.

Cavallo F, Mirza K, de Mateo S, Miglietta L, Rodriguez-Manzano J, Nikolic K Biosensors (Basel). 2022; 12(7).

PMID: 35884340 PMC: 9313270. DOI: 10.3390/bios12070537.

Modular micro-PCR system for the onsite rapid diagnosis of COVID-19.

Nguyen P, Wang M, Ann Maria N, Li A, Tan H, Xiong G Microsyst Nanoeng. 2022; 8:82.

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