Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 May 26

PMID 37232905

Authors

Francesca Costantini

Nicola Lovecchio

Manasa Nandimandalam

Ariana Manglli

Francesco Faggioli

Mara Biasin

Cesare Manetti

Pio Federico Roversi

Augusto Nascetti

Giampiero de Cesare

Domenico Caputo

Affiliations

Soon will be listed here.

Abstract

Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG) obtained using metal thin-film deposition. By using a microwell plate optically coupled with the SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has been carried out in the developed LoC-PCR device. The limit of detection and time of analysis for the detection of the two viruses by using the LoC-PCR were compared with those achieved by standard equipment. The results showed that the two systems can detect the same concentration of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard thermocycler, with the advantage of the portability, leading to a point-of-care device for several diagnostic applications.

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