Protective Effects of Egyptian Cloudy Apple Juice and Apple Peel Extract on Lipid Peroxidation, Antioxidant Enzymes and Inflammatory Status in Diabetic Rat Pancreas

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2016 Jan 13

PMID 26753525

Citations 17

Authors

Samah M Fathy

Ehab A Drees

Affiliations

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Abstract

Background: Apples possess rich content of varied polyphenolic compounds showing a variety of biological activities that may ascribe to worthy effects against some chronic diseases. The present study was designed to assess the protective effects of the cloudy apple juice (CAJ) and apple peel extract (APE) of Egyptian Anna apple on the complications in experimental diabetes.

Materials And Methods: Four groups were studied. Diabetes was induced by a single dose of streptozotocin (STZ) to only three groups of albino Wistar rats. Two of the diabetic groups received either CAJ or APE for 21 days. At the end of the study, lipid profile parameters were measured in serum while lipid peroxidation (LPO) level, antioxidant enzyme activities and inflammatory markers were evaluated in pancreas tissue samples. The gas chromatography-mass spectrometry (GC-MS) analysis of phenolic compounds found in CAJ and APE was carried out. Moreover, total phenolic content of CAJ and APE were measured.

Results: The significant increase of blood glucose level, serum total cholesterol (TC), triglycerides (TG), low- density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein (VLDL) levels, in addition to tissue malondialdehyde (MDA), nuclear factor kappa B (NF-kB), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), interleukin 8 (IL-8) levels, but a significant decrease in high-density lipoprotein cholesterol (HDL-C), and the activity of pancreatic antioxidant enzymes were the remarkably parameters observed in diabetic control rats. Dissimilarly, oral supplementation of 15 ml/kg CAJ and 1 g/kg APE for 21 days resulted in a significant decrease in fasting blood glucose, serum TC, TG, LDL-C, VLDL-C and tissue MDA, NF-kB, TNF-α, IL-6, IL-8 levels coupled with a significant elevation of HDL-C and antioxidant enzymes' activity when compared with diabetic control animals.

Conclusions: The results indicate that Egyptian CAJ and APE supplementation may have protective effects against deleterious complications of diabetes mellitus.

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References

FRIEDEWALD W, Levy R, Fredrickson D . Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18(6):499-502. View

Atli T, Keven K, Avci A, Kutlay S, Turkcapar N, Varli M . Oxidative stress and antioxidant status in elderly diabetes mellitus and glucose intolerance patients. Arch Gerontol Geriatr. 2004; 39(3):269-75. DOI: 10.1016/j.archger.2004.04.065. View

Mokhtari D, Li T, Lu T, Welsh N . Effects of Imatinib Mesylate (Gleevec) on human islet NF-kappaB activation and chemokine production in vitro. PLoS One. 2011; 6(9):e24831. PMC: 3173488. DOI: 10.1371/journal.pone.0024831. View

Turkyilmaz M, Tagi S, Dereli U, Ozkan M . Effects of various pressing programs and yields on the antioxidant activity, antimicrobial activity, phenolic content and colour of pomegranate juices. Food Chem. 2013; 138(2-3):1810-8. DOI: 10.1016/j.foodchem.2012.11.100. View

Sadek K, Shaheen H . Biochemical efficacy of vitamin D in ameliorating endocrine and metabolic disorders in diabetic rats. Pharm Biol. 2013; 52(5):591-6. DOI: 10.3109/13880209.2013.854812. View

Salvamani S, Gunasekaran B, Shaharuddin N, Ahmad S, Shukor M . Antiartherosclerotic effects of plant flavonoids. Biomed Res Int. 2014; 2014:480258. PMC: 4058282. DOI: 10.1155/2014/480258. View

Kim M, Ryu G, Chung J, Sim S, Min D, Rhie D . Protective effects of epicatechin against the toxic effects of streptozotocin on rat pancreatic islets: in vivo and in vitro. Pancreas. 2003; 26(3):292-9. DOI: 10.1097/00006676-200304000-00014. View

Abbott Galvao Ururahy M, Costa de Souza K, Oliveira Y, Loureiro M, da Silva H, Freire-Neto F . Association of polymorphisms in IL6 gene promoter region with type 1 diabetes and increased albumin-to-creatinine ratio. Diabetes Metab Res Rev. 2014; 31(5):500-6. DOI: 10.1002/dmrr.2621. View

Ueda Yaochite J, Caliari-Oliveira C, Davanso M, Carlos D, Malmegrim K, de Barros Cardoso C . Dynamic changes of the Th17/Tc17 and regulatory T cell populations interfere in the experimental autoimmune diabetes pathogenesis. Immunobiology. 2012; 218(3):338-52. DOI: 10.1016/j.imbio.2012.05.010. View

10.

Hyson D . A comprehensive review of apples and apple components and their relationship to human health. Adv Nutr. 2012; 2(5):408-20. PMC: 3183591. DOI: 10.3945/an.111.000513. View

11.

Aliciguzel Y, Ozen I, Aslan M, Karayalcin U . Activities of xanthine oxidoreductase and antioxidant enzymes in different tissues of diabetic rats. J Lab Clin Med. 2003; 142(3):172-7. DOI: 10.1016/S0022-2143(03)00110-0. View

12.

Salah N, Miller N, Paganga G, Tijburg L, Bolwell G . Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys. 1995; 322(2):339-46. DOI: 10.1006/abbi.1995.1473. View

13.

de Ferranti S, Mozaffarian D . The perfect storm: obesity, adipocyte dysfunction, and metabolic consequences. Clin Chem. 2008; 54(6):945-55. DOI: 10.1373/clinchem.2007.100156. View

14.

Goldberg R . Lipid disorders in diabetes. Diabetes Care. 1981; 4(5):561-72. DOI: 10.2337/diacare.4.5.561. View

15.

Maritim A, Sanders R, Watkins 3rd J . Effects of alpha-lipoic acid on biomarkers of oxidative stress in streptozotocin-induced diabetic rats. J Nutr Biochem. 2003; 14(5):288-94. DOI: 10.1016/s0955-2863(03)00036-6. View

16.

Mysliwiec M, Balcerska A, Zorena K, Mysliwska J, Lipowski P, Raczynska K . The role of vascular endothelial growth factor, tumor necrosis factor alpha and interleukin-6 in pathogenesis of diabetic retinopathy. Diabetes Res Clin Pract. 2007; 79(1):141-6. DOI: 10.1016/j.diabres.2007.07.011. View

17.

Shin D, Kim K, Young Chung H, Yoon S, Moon J . Effect of sinapic acid against dimethylnitrosamine-induced hepatic fibrosis in rats. Arch Pharm Res. 2013; 36(5):608-18. DOI: 10.1007/s12272-013-0033-6. View

18.

Hectors T, Vanparys C, van der Ven K, Martens G, Jorens P, Van Gaal L . Environmental pollutants and type 2 diabetes: a review of mechanisms that can disrupt beta cell function. Diabetologia. 2011; 54(6):1273-90. DOI: 10.1007/s00125-011-2109-5. View

19.

Boyer J, Liu R . Apple phytochemicals and their health benefits. Nutr J. 2004; 3:5. PMC: 442131. DOI: 10.1186/1475-2891-3-5. View

20.

Sun J, Chu Y, Wu X, Liu R . Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem. 2002; 50(25):7449-54. DOI: 10.1021/jf0207530. View