Multimodal Optical Imaging System for in Vivo Investigation of Cerebral Oxygen Delivery and Energy Metabolism

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2015 Dec 30

PMID 26713212

Citations 25

Authors

Mohammad A Yaseen

Vivek J Srinivasan

Iwona Gorczynska

James G Fujimoto

David A Boas

Sava Sakadzic

Affiliations

Soon will be listed here.

Abstract

Improving our understanding of brain function requires novel tools to observe multiple physiological parameters with high resolution in vivo. We have developed a multimodal imaging system for investigating multiple facets of cerebral blood flow and metabolism in small animals. The system was custom designed and features multiple optical imaging capabilities, including 2-photon and confocal lifetime microscopy, optical coherence tomography, laser speckle imaging, and optical intrinsic signal imaging. Here, we provide details of the system's design and present in vivo observations of multiple metrics of cerebral oxygen delivery and energy metabolism, including oxygen partial pressure, microvascular blood flow, and NADH autofluorescence.

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