Simultaneous Imaging of Cerebral Partial Pressure of Oxygen and Blood Flow During Functional Activation and Cortical Spreading Depression

Overview

Journal Appl Opt

Date 2009 Apr 3

PMID 19340106

Citations 31

Authors

Sava Sakadzic

Shuai Yuan

Ergin Dilekoz

Svetlana Ruvinskaya

Sergei A Vinogradov

Cenk Ayata

David A Boas

Affiliations

Soon will be listed here.

Abstract

We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.

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