A Thermostable Sequence-specific Endonuclease from Thermus Aquaticus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Feb 1

PMID 265518

Citations 61

Authors

S Sato

C A Hutchinson 3rd

J I Harris

Affiliations

Soon will be listed here.

Abstract

A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and bacteriophage psiX174 RF DNA at 10 sites. The enzyme is active at temperatures up to 70 degrees. The cleavage sites on psiX174 RF DNA have been mapped. The sequence recognized and cleaved by Taq I has been shown to be the symmetrical tetranucleotide: (formula: see text).

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