DNA Sequence of the AraC Regulatory Gene from Escherichia Coli B/r

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Nov 25

PMID 7008027

Citations 26

Authors

C G Miyada

A H Horwitz

L G Cass

J Timko

G Wilcox

Affiliations

Soon will be listed here.

Abstract

The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation in araC, delta(araCO)719, removes all but the last 22 codons of the gene.

Citing Articles

Comparison between Effects of Retroactivity and Resource Competition upon Change in Downstream Reporter Genes of Synthetic Genetic Circuits.

Moriya T, Yamaoka T, Wakayama Y, Ayukawa S, Zhang Z, Yamamura M Life (Basel). 2019; 9(1).

PMID: 30917535 PMC: 6463139. DOI: 10.3390/life9010030.

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

Arac/XylS family of transcriptional regulators.

Gallegos M, Schleif R, Bairoch A, Hofmann K, Ramos J Microbiol Mol Biol Rev. 1997; 61(4):393-410.

PMID: 9409145 PMC: 232617. DOI: 10.1128/mmbr.61.4.393-410.1997.

Identification of DNA sequences recognized by VirF, the transcriptional activator of the Yersinia yop regulon.

Wattiau P, Cornelis G J Bacteriol. 1994; 176(13):3878-84.

PMID: 8021169 PMC: 205584. DOI: 10.1128/jb.176.13.3878-3884.1994.

Many gene-regulatory proteins appear to have a similar alpha-helical fold that binds DNA and evolved from a common precursor.

Ohlendorf D, Anderson W, Matthews B J Mol Evol. 1983; 19(2):109-14.

PMID: 6571216 DOI: 10.1007/BF02300748.

References

ENGLESBERG E, Squires C, Meronk Jr F . The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969; 62(4):1100-7. PMC: 223620. DOI: 10.1073/pnas.62.4.1100. View

Irr J, ENGLESBERG E . Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r. Genetics. 1970; 65(1):27-39. PMC: 1212430. DOI: 10.1093/genetics/65.1.27. View

Lee N, Wilcox G, Gielow W, Arnold J, Cleary P, ENGLESBERG E . In vitro activation of the transcription of araBAD operon by araC activator. Proc Natl Acad Sci U S A. 1974; 71(3):634-8. PMC: 388066. DOI: 10.1073/pnas.71.3.634. View

Wilcox G, Clemetson K, Cleary P, ENGLESBERG E . Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli. J Mol Biol. 1974; 85(4):589-602. DOI: 10.1016/0022-2836(74)90317-9. View

Wilcox G, Boulter J, Lee N . Direction of transcription of the regulatory gene araC in Escherichia coli B-r. Proc Natl Acad Sci U S A. 1974; 71(9):3635-9. PMC: 433830. DOI: 10.1073/pnas.71.9.3635. View

ENGLESBERG E, Wilcox G . Regulation: positive control. Annu Rev Genet. 1974; 8:219-42. DOI: 10.1146/annurev.ge.08.120174.001251. View

Casadaban M . Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol. 1976; 104(3):557-66. DOI: 10.1016/0022-2836(76)90120-0. View

Smith H, Birnstiel M . A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976; 3(9):2387-98. PMC: 343093. DOI: 10.1093/nar/3.9.2387. View

Murray K, Hughes S, Brown J, Bruce S . Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis. Biochem J. 1976; 159(2):317-22. PMC: 1164119. DOI: 10.1042/bj1590317. View

10.

Sato S, Hutchinson 3rd C, Harris J . A thermostable sequence-specific endonuclease from Thermus aquaticus. Proc Natl Acad Sci U S A. 1977; 74(2):542-6. PMC: 392326. DOI: 10.1073/pnas.74.2.542. View

11.

SANGER F, Coulson A . The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978; 87(1):107-10. DOI: 10.1016/0014-5793(78)80145-8. View

12.

Smith B, Schleif R . Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12. J Biol Chem. 1978; 253(19):6931-3. View

13.

Greenfield L, Boone T, Wilcox G . DNA sequence of the araBAD promoter in Escherichia coli B/r. Proc Natl Acad Sci U S A. 1978; 75(10):4724-8. PMC: 336192. DOI: 10.1073/pnas.75.10.4724. View

14.

Norgard M, Emigholz K, Monahan J . Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside. J Bacteriol. 1979; 138(1):270-2. PMC: 218271. DOI: 10.1128/jb.138.1.270-272.1979. View

15.

Johnson R, Walseth T . The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases. Adv Cyclic Nucleotide Res. 1979; 10:135-67. View

16.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

17.

Queen C, Korn L . Computer analysis of nucleic acids and proteins. Methods Enzymol. 1980; 65(1):595-609. DOI: 10.1016/s0076-6879(80)65062-9. View