Functionalized Nanofiber Meshes Enhance Immunosorbent Assays

Overview

Journal Anal Chem

Specialty Chemistry

Date 2015 Nov 10

PMID 26551162

Citations 5

Authors

Joseph S Hersey

Amit Meller

Mark W Grinstaff

Affiliations

Soon will be listed here.

Abstract

Three-dimensional substrates with high surface-to-volume ratios and subsequently large protein binding capacities are of interest for advanced immunosorbent assays utilizing integrated microfluidics and nanosensing elements. A library of bioactive and antifouling electrospun nanofiber substrates, which are composed of high-molecular-weight poly(oxanorbornene) derivatives, is described. Specifically, a set of copolymers are synthesized from three 7-oxanorbornene monomers to create a set of water insoluble copolymers with both biotin (bioactive) and triethylene glycol (TEG) (antifouling) functionality. Porous three-dimensional nanofiber meshes are electrospun from these copolymers with the ability to specifically bind streptavidin while minimizing the nonspecific binding of other proteins. Fluorescently labeled streptavidin is used to quantify the streptavidin binding capacity of each mesh type through confocal microscopy. A simplified enzyme-linked immunosorbent assay (ELISA) is presented to assess the protein binding capabilities and detection limits of these nanofiber meshes under both static conditions (26 h) and flow conditions (1 h) for a model target protein (i.e., mouse IgG) using a horseradish peroxidase (HRP) colorimetric assay. Bioactive and antifouling nanofiber meshes outperform traditional streptavidin-coated polystyrene plates under flow, validating their use in future advanced immunosorbent assays and their compatibility with microfluidic-based biosensors.

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References

Wanunu M, Sutin J, McNally B, Chow A, Meller A . DNA translocation governed by interactions with solid-state nanopores. Biophys J. 2008; 95(10):4716-25. PMC: 2576395. DOI: 10.1529/biophysj.108.140475. View

Wang J . Electrochemical biosensors: towards point-of-care cancer diagnostics. Biosens Bioelectron. 2005; 21(10):1887-92. DOI: 10.1016/j.bios.2005.10.027. View

Richter M, Chakrabarti A, Ruttekolk I, Wiesner B, Beyermann M, Brock R . Multivalent design of apoptosis-inducing bid-BH3 peptide-oligosaccharides boosts the intracellular activity at identical overall peptide concentrations. Chemistry. 2012; 18(52):16708-15. DOI: 10.1002/chem.201202276. View

Matlock-Colangelo L, Baeumner A . Biologically inspired nanofibers for use in translational bioanalytical systems. Annu Rev Anal Chem (Palo Alto Calif). 2014; 7:23-42. DOI: 10.1146/annurev-anchem-071213-020035. View

Goryacheva I, De Saeger S, Eremin S, Van Peteghem C . Immunochemical methods for rapid mycotoxin detection: evolution from single to multiple analyte screening: a review. Food Addit Contam. 2007; 24(10):1169-83. DOI: 10.1080/02652030701557179. View

Zhou F, Wang M, Yuan L, Cheng Z, Wu Z, Chen H . Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection. Analyst. 2012; 137(8):1779-84. DOI: 10.1039/c2an16257a. View

Matlock-Colangelo L, Baeumner A . Recent progress in the design of nanofiber-based biosensing devices. Lab Chip. 2012; 12(15):2612-20. DOI: 10.1039/c2lc21240d. View

Wathier M, Stoddart S, Sheehy M, Grinstaff M . Acidic polysaccharide mimics via ring-opening metathesis polymerization. J Am Chem Soc. 2010; 132(45):15887-9. DOI: 10.1021/ja106488h. View

Schneider C, Rasband W, Eliceiri K . NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012; 9(7):671-5. PMC: 5554542. DOI: 10.1038/nmeth.2089. View

10.

Green N . Avidin. Adv Protein Chem. 1975; 29:85-133. DOI: 10.1016/s0065-3233(08)60411-8. View

11.

Lee I, Luo X, Huang J, Cui X, Yun M . Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors. Biosensors (Basel). 2015; 2(2):205-20. PMC: 4263570. DOI: 10.3390/bios2020205. View

12.

Wathier M, Lakin B, Bansal P, Stoddart S, Snyder B, Grinstaff M . A large-molecular-weight polyanion, synthesized via ring-opening metathesis polymerization, as a lubricant for human articular cartilage. J Am Chem Soc. 2013; 135(13):4930-3. DOI: 10.1021/ja400695h. View

13.

Green N . AVIDIN. 1. THE USE OF (14-C)BIOTIN FOR KINETIC STUDIES AND FOR ASSAY. Biochem J. 1963; 89:585-91. PMC: 1202466. DOI: 10.1042/bj0890585. View

14.

Lu T, Chen X, Shi Q, Wang Y, Zhang P, Jing X . The immobilization of proteins on biodegradable fibers via biotin-streptavidin bridges. Acta Biomater. 2008; 4(6):1770-7. DOI: 10.1016/j.actbio.2008.05.006. View

15.

Patel A, Li S, Yuan J, Wei Y . In situ encapsulation of horseradish peroxidase in electrospun porous silica fibers for potential biosensor applications. Nano Lett. 2006; 6(5):1042-6. DOI: 10.1021/nl0604560. View

16.

Squires A, Hersey J, Grinstaff M, Meller A . A nanopore-nanofiber mesh biosensor to control DNA translocation. J Am Chem Soc. 2013; 135(44):16304-7. PMC: 4039743. DOI: 10.1021/ja408685x. View