Tumorsphere As an Effective in Vitro Platform for Screening Anti-cancer Stem Cell Drugs

Overview

Journal Oncotarget

Specialty Oncology

Date 2015 Nov 4

PMID 26527320

Citations 98

Authors

Che-Hsin Lee

Cheng-Chia Yu

Bing-Yen Wang

Wen-Wei Chang

Affiliations

Soon will be listed here.

Abstract

Cancer stem cells (CSCs) are a sub-population of cells within cancer tissues with tumor initiation, drug resistance and metastasis properties. CSCs also have been considered as the main cause of cancer recurrence. Targeting CSCs have been suggested as the key for successful treatment against cancer. Tumorsphere cultivation is based on culturing cancer cells onto ultralow attachment surface in serum-free media under the supplementation with growth factors such as epidermal growth factor and basic fibroblast growth factor. Tumorsphere cultivation is widely used to analyze the self-renewal capability of CSCs and to enrich these cells from bulk cancer cells. This method also provides a reliable platform for screening potential anti-CSC agents. The in vitro anti-proliferation activity of potential agents selected from tumorsphere assay is more translatable into in vivo anti-tumorigenic activity compared with general monolayer culture. Tumorsphere assay can also measure the outcome of clinical trials for potential anti-cancer agents. In addition, tumorsphere assay may be a promising strategy in the innovation of future cancer therapeutica and may help in the screening of anti-cancer small-molecule chemicals.

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References

Zhan H, Xu J, Wu D, Zhang T, Hu S . Pancreatic cancer stem cells: new insight into a stubborn disease. Cancer Lett. 2014; 357(2):429-37. DOI: 10.1016/j.canlet.2014.12.004. View

Zhou J, Zhang H, Gu P, Margolick J, Yin D, Zhang Y . Cancer stem/progenitor cell active compound 8-quinolinol in combination with paclitaxel achieves an improved cure of breast cancer in the mouse model. Breast Cancer Res Treat. 2008; 115(2):269-77. PMC: 3320107. DOI: 10.1007/s10549-008-0072-8. View

Hayashi T, Ding Q, Kuwahata T, Maeda K, Miyazaki Y, Matsubara S . Interferon-alpha modulates the chemosensitivity of CD133-expressing pancreatic cancer cells to gemcitabine. Cancer Sci. 2012; 103(5):889-96. PMC: 7659312. DOI: 10.1111/j.1349-7006.2012.02235.x. View

Liao J, Qian F, Tchabo N, Mhawech-Fauceglia P, Beck A, Qian Z . Ovarian cancer spheroid cells with stem cell-like properties contribute to tumor generation, metastasis and chemotherapy resistance through hypoxia-resistant metabolism. PLoS One. 2014; 9(1):e84941. PMC: 3883678. DOI: 10.1371/journal.pone.0084941. View

Chang W, Kuan Y, Chen M, Lin S, Lee C . Tracking of mouse breast cancer stem-like cells with Salmonella. Exp Biol Med (Maywood). 2012; 237(10):1189-96. DOI: 10.1258/ebm.2012.012063. View

Hermann P, Bhaskar S, Cioffi M, Heeschen C . Cancer stem cells in solid tumors. Semin Cancer Biol. 2010; 20(2):77-84. DOI: 10.1016/j.semcancer.2010.03.004. View

Jackson M, Hassiotou F, Nowak A . Glioblastoma stem-like cells: at the root of tumor recurrence and a therapeutic target. Carcinogenesis. 2014; 36(2):177-85. DOI: 10.1093/carcin/bgu243. View

Dangles-Marie V, Pocard M, Richon S, Weiswald L, Assayag F, Saulnier P . Establishment of human colon cancer cell lines from fresh tumors versus xenografts: comparison of success rate and cell line features. Cancer Res. 2007; 67(1):398-407. DOI: 10.1158/0008-5472.CAN-06-0594. View

Crous A, Abrahamse H . Lung cancer stem cells and low-intensity laser irradiation: a potential future therapy?. Stem Cell Res Ther. 2013; 4(5):129. PMC: 3854767. DOI: 10.1186/scrt340. View

10.

Cao D, Kishida S, Huang P, Mu P, Tsubota S, Mizuno M . A new tumorsphere culture condition restores potentials of self-renewal and metastasis of primary neuroblastoma in a mouse neuroblastoma model. PLoS One. 2014; 9(1):e86813. PMC: 3899333. DOI: 10.1371/journal.pone.0086813. View

11.

Mani S, Guo W, Liao M, Eaton E, Ayyanan A, Zhou A . The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell. 2008; 133(4):704-15. PMC: 2728032. DOI: 10.1016/j.cell.2008.03.027. View

12.

Oishi N, Yamashita T, Kaneko S . Molecular biology of liver cancer stem cells. Liver Cancer. 2014; 3(2):71-84. PMC: 4057789. DOI: 10.1159/000343863. View

13.

Nonaka M, Yawata T, Takemura M, Higashi Y, Nakai E, Shimizu K . Elevated cell invasion in a tumor sphere culture of RSV-M mouse glioma cells. Neurol Med Chir (Tokyo). 2015; 55(1):60-70. PMC: 4533394. DOI: 10.2176/nmc.oa.2014-0067. View

14.

Sharpe B, Beresford M, Bowen R, Mitchard J, Chalmers A . Searching for prostate cancer stem cells: markers and methods. Stem Cell Rev Rep. 2013; 9(5):721-30. DOI: 10.1007/s12015-013-9453-4. View

15.

Hanahan D, Weinberg R . Hallmarks of cancer: the next generation. Cell. 2011; 144(5):646-74. DOI: 10.1016/j.cell.2011.02.013. View

16.

Kim S, Alexander C . Tumorsphere assay provides more accurate prediction of in vivo responses to chemotherapeutics. Biotechnol Lett. 2013; 36(3):481-8. PMC: 3966961. DOI: 10.1007/s10529-013-1393-1. View

17.

Tomao F, Papa A, Strudel M, Rossi L, Lo Russo G, Benedetti Panici P . Investigating molecular profiles of ovarian cancer: an update on cancer stem cells. J Cancer. 2014; 5(5):301-10. PMC: 3982176. DOI: 10.7150/jca.8610. View

18.

Folkins C, Shaked Y, Man S, Tang T, Lee C, Zhu Z . Glioma tumor stem-like cells promote tumor angiogenesis and vasculogenesis via vascular endothelial growth factor and stromal-derived factor 1. Cancer Res. 2009; 69(18):7243-51. PMC: 3409689. DOI: 10.1158/0008-5472.CAN-09-0167. View

19.

Hashimoto N, Tsunedomi R, Yoshimura K, Watanabe Y, Hazama S, Oka M . Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs. BMC Cancer. 2014; 14:722. PMC: 4190290. DOI: 10.1186/1471-2407-14-722. View

20.

Chang W, Lin R, Yu J, Chang W, Fu C, Lai A . The expression and significance of insulin-like growth factor-1 receptor and its pathway on breast cancer stem/progenitors. Breast Cancer Res. 2013; 15(3):R39. PMC: 3706809. DOI: 10.1186/bcr3423. View