The Y3** NcRNA Promotes the 3' End Processing of Histone MRNAs

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2015 Oct 8

PMID 26443846

Citations 27

Authors

Marcel Kohn

Christian Ihling

Andrea Sinz

Knut Krohn

Stefan Huttelmaier

Affiliations

Soon will be listed here.

Abstract

We demonstrate that the Y3/Y3** noncoding RNAs (ncRNAs) bind to the CPSF (cleavage and polyadenylation specificity factor) and that Y3** associates with the 3' untranslated region (UTR) of histone pre-mRNAs. The depletion of Y3** impairs the 3' end processing of histone pre-mRNAs as well as the formation and protein dynamics of histone locus bodies (HLBs), the site of histone mRNA synthesis and processing. HLB morphology is also disturbed by knockdown of the CPSF but not the U7-snRNP components. In conclusion, we propose that the Y3** ncRNA promotes the 3' end processing of histone pre-mRNAs by enhancing the recruitment of the CPSF to histone pre-mRNAs at HLBs.

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