Sequence Requirements for Branch Formation in a Group II Self-splicing Intron

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Jan 11

PMID 2643082

Citations 4

Authors

R Altura

B Rymond

B Seraphin

M Rosbash

Affiliations

Soon will be listed here.

Abstract

Evidence is presented for the existence of a specific intron-intron interaction, necessary for the formation of the branched product in the self-splicing reaction of a group II yeast mitochondrial intron. Trans-splicing reactions involving two RNA molecules (5' exon with covalently linked regions of intron and intron with covalently linked 3' exon) show that the presence of portions of intron domain I on the 5' molecule is necessary for the formation of branched products which are not seen with shorter 5' molecules. Modification/interference reactions show regions necesary for branch-formation and support a major role for specific regions of intron domain I. Further experiments, utilizing a truncated 3' molecule that is missing the conserved branchpoint nucleotide, indicate that domain VI may be required for a successful domain I interaction. A model for the formation of a proper branched structure includes implications for both cis and trans configurations.

Citing Articles

Stereochemical selectivity of group II intron splicing, reverse splicing, and hydrolysis reactions.

Podar M, Perlman P, Padgett R Mol Cell Biol. 1995; 15(8):4466-78.

PMID: 7542746 PMC: 230686. DOI: 10.1128/MCB.15.8.4466.

Self-splicing of the mobile group II intron of the filamentous fungus Podospora anserina (COI I1) in vitro.

Schmidt U, Riederer B, Morl M, Schmelzer C, Stahl U EMBO J. 1990; 9(7):2289-98.

PMID: 2162769 PMC: 551955. DOI: 10.1002/j.1460-2075.1990.tb07400.x.

Structural requirements for selection of 5'- and 3' splice sites of group II introns.

Wallasch C, Morl M, Niemer I, Schmelzer C Nucleic Acids Res. 1991; 19(12):3307-14.

PMID: 2062646 PMC: 328327. DOI: 10.1093/nar/19.12.3307.

Nucleotide sequence of the COX1 gene in Kluyveromyces lactis mitochondrial DNA: evidence for recent horizontal transfer of a group II intron.

Hardy C, Clark-Walker G Curr Genet. 1991; 20(1-2):99-114.

PMID: 1657415 DOI: 10.1007/BF00312772.

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