Methane-rich Saline Attenuates Ischemia/reperfusion Injury of Abdominal Skin Flaps in Rats Via Regulating Apoptosis Level

Overview

Journal BMC Surg

Publisher Biomed Central

Specialty General Surgery

Date 2015 Aug 1

PMID 26228913

Citations 29

Authors

Kexin Song

Mingzi Zhang

Jianqiang Hu

Yunqi Liu

Yifang Liu

Youbin Wang

Xuemei Ma

Affiliations

Soon will be listed here.

Abstract

Background: In plastic surgery, skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs. Methane gas has been reported to be a new therapeutic gas for attenuating I/R injury. In this study, we assessed the effects of methane-rich saline (MRS) in regulating apoptosis on skin flap I/R injury.

Methods: Male Sprague-Dawley rats, 6-8 weeks old, were divided randomly into three groups: one sham surgery group (SH) and two surgery groups. After undergoing 6 h of I/R management of an abdominal skin flap, surgery groups were treated with physiological saline (I/R-P) or methane-rich saline (I/R-M). On the 3rd postoperative day, a laser Doppler flowmeter was used to measure flap blood supply, and hematoxylin and eosin (H&E) staining was used to observe morphological changes. TdT-mediated dUTP-X nick end labeling (TUNEL) staining was also used to observe early apoptosis and is presented as the percentage of TUNEL-positive cells. Moreover, pASK-1, pJNK, Bcl-2 and Bax were detected by immunohistochemical technology. Caspase-3 activity was also measured to evaluate the effects of MRS.

Results: Compared to the I/R-P group, the flaps in the I/R-M group presented a larger survival area and better blood perfusion with less inflammatory infiltration and cell apoptosis, a higher expression of Bcl-2, a lower expression of pASK-1, pJNK and Bax, and a lower caspase-3 activity.

Conclusion: According to the results, MRS attenuated I/R injury by regulating apoptosis and has the potential to be applied as a new therapy for improving skin flap survival.

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