A Putative, Novel Coli Surface Antigen 8B (CS8B) of Enterotoxigenic Escherichia Coli

Overview

Journal Pathog Dis

Publisher Oxford University Press

Specialties Infectious Diseases
Microbiology

Date 2015 Jul 19

PMID 26187892

Citations 2

Authors

Samuel M Njoroge

Christine J Boinett

Laure F Made

Tom T Ouko

Eric M Fevre

Nicholas R Thomson

Samuel Kariuki

Affiliations

Soon will be listed here.

Abstract

Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described.

Citing Articles

Clinical and Molecular Analysis of ST11-K47 Carbapenem-Resistant Hypervirulent : A Strain Causing Liver Abscess.

Cai Z, Jia T, Pu M, Zhang S, Zhang J, Geng R Pathogens. 2022; 11(6).

PMID: 35745510 PMC: 9227846. DOI: 10.3390/pathogens11060657.

Chaperone-Usher Pili Loci of Colonization Factor-Negative Human Enterotoxigenic .

Del Canto F, ORyan M, Pardo M, Torres A, Gutierrez D, Cadiz L Front Cell Infect Microbiol. 2017; 6:200.

PMID: 28111618 PMC: 5216030. DOI: 10.3389/fcimb.2016.00200.

References

Begum Y, Baby N, Faruque A, Jahan N, Cravioto A, Svennerholm A . Shift in phenotypic characteristics of enterotoxigenic Escherichia coli (ETEC) isolated from diarrheal patients in Bangladesh. PLoS Negl Trop Dis. 2014; 8(7):e3031. PMC: 4102457. DOI: 10.1371/journal.pntd.0003031. View

Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream M . Artemis: sequence visualization and annotation. Bioinformatics. 2000; 16(10):944-5. DOI: 10.1093/bioinformatics/16.10.944. View

Tamura K, Nei M . Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993; 10(3):512-26. DOI: 10.1093/oxfordjournals.molbev.a040023. View

Nataro J, Kaper J . Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998; 11(1):142-201. PMC: 121379. DOI: 10.1128/CMR.11.1.142. View

Aanensen D, Spratt B . The multilocus sequence typing network: mlst.net. Nucleic Acids Res. 2005; 33(Web Server issue):W728-33. PMC: 1160176. DOI: 10.1093/nar/gki415. View

Qadri F, Svennerholm A, Faruque A, Sack R . Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005; 18(3):465-83. PMC: 1195967. DOI: 10.1128/CMR.18.3.465-483.2005. View

Carattoli A, Bertini A, Villa L, Falbo V, Hopkins K, Threlfall E . Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005; 63(3):219-28. DOI: 10.1016/j.mimet.2005.03.018. View

Turner S, Scott-Tucker A, Cooper L, Henderson I . Weapons of mass destruction: virulence factors of the global killer enterotoxigenic Escherichia coli. FEMS Microbiol Lett. 2006; 263(1):10-20. DOI: 10.1111/j.1574-6968.2006.00401.x. View

Ohtsubo Y, Ikeda-Ohtsubo W, Nagata Y, Tsuda M . GenomeMatcher: a graphical user interface for DNA sequence comparison. BMC Bioinformatics. 2008; 9:376. PMC: 2553346. DOI: 10.1186/1471-2105-9-376. View

10.

Rodas C, Iniguez V, Qadri F, Wiklund G, Svennerholm A, Sjoling A . Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins. J Clin Microbiol. 2009; 47(4):1218-20. PMC: 2668350. DOI: 10.1128/JCM.00316-09. View

11.

Fleckenstein J, Hardwidge P, Munson G, Rasko D, Sommerfelt H, Steinsland H . Molecular mechanisms of enterotoxigenic Escherichia coli infection. Microbes Infect. 2009; 12(2):89-98. PMC: 10647112. DOI: 10.1016/j.micinf.2009.10.002. View

12.

Mazariego-Espinosa K, Cruz A, Ledesma M, Ochoa S, Xicohtencatl-Cortes J . Longus, a type IV pilus of enterotoxigenic Escherichia coli, is involved in adherence to intestinal epithelial cells. J Bacteriol. 2010; 192(11):2791-800. PMC: 2876479. DOI: 10.1128/JB.01595-09. View

13.

Zerbino D . Using the Velvet de novo assembler for short-read sequencing technologies. Curr Protoc Bioinformatics. 2010; Chapter 11:Unit 11.5. PMC: 2952100. DOI: 10.1002/0471250953.bi1105s31. View

14.

Nada R, Shaheen H, Khalil S, Mansour A, El-Sayed N, Touni I . Discovery and phylogenetic analysis of novel members of class b enterotoxigenic Escherichia coli adhesive fimbriae. J Clin Microbiol. 2011; 49(4):1403-10. PMC: 3122862. DOI: 10.1128/JCM.02006-10. View

15.

Sievers F, Wilm A, Dineen D, Gibson T, Karplus K, Li W . Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 2011; 7:539. PMC: 3261699. DOI: 10.1038/msb.2011.75. View

16.

Yuen A, Kolappan S, Ng D, Craig L . Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli. Mol Microbiol. 2013; 90(4):898-918. DOI: 10.1111/mmi.12407. View

17.

Taniguchi T, Akeda Y, Haba A, Yasuda Y, Yamamoto K, Honda T . Gene cluster for assembly of pilus colonization factor antigen III of enterotoxigenic Escherichia coli. Infect Immun. 2001; 69(9):5864-73. PMC: 98705. DOI: 10.1128/IAI.69.9.5864-5873.2001. View

18.

Shaheen H, Kamal K, Wasfy M, El-Ghorab N, Lowe B, Steffen R . Phenotypic diversity of enterotoxigenic Escherichia coli (ETEC) isolated from cases of travelers' diarrhea in Kenya. Int J Infect Dis. 2003; 7(1):35-8. DOI: 10.1016/s1201-9712(03)90040-3. View