Application of CRISPR/Cas9 for Biomedical Discoveries

Overview

Journal Cell Biosci

Publisher Biomed Central

Specialty Biology

Date 2015 Jul 3

PMID 26137216

Citations 19

Authors

Sean M Riordan

Daniel P Heruth

Li Q Zhang

Shui Qing Ye

Affiliations

Soon will be listed here.

Abstract

The Clustered Regions of Interspersed Palindromic Repeats-Cas9 (CRISPR/Cas9), a viral defense system found in bacteria and archaea, has emerged as a tour de force genome editing tool. The CRISPR/Cas9 system is much easier to customize and optimize because the site selection for DNA cleavage is guided by a short sequence of RNA rather than an engineered protein as in the systems of zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and meganucleases. Although it still suffers from some off-target effects, the CRISPR/Cas9 system has been broadly and successfully applied for biomedical discoveries in a number of areas. In this review, we present a brief history and development of the CRISPR system and focus on the application of this genome editing technology for biomedical discoveries. We then present concise concluding remarks and future directions for this fast moving field.

Citing Articles

Revitalizing oral cancer research: Crispr-Cas9 technology the promise of genetic editing.

S V S, Augustine D, Mushtaq S, Ali Baeshen H, Ashi H, Hassan R Front Oncol. 2024; 14:1383062.

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Identification of Genes Regulating Hepatocyte Injury by a Genome-Wide CRISPR-Cas9 Screen.

Shortt K, Heruth D Methods Mol Biol. 2022; 2544:227-251.

PMID: 36125723 DOI: 10.1007/978-1-0716-2557-6_17.

CRISPR-Cas systems for diagnosing infectious diseases.

Kostyusheva A, Brezgin S, Babin Y, Vasilyeva I, Glebe D, Kostyushev D Methods. 2021; 203:431-446.

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Improvements in Gene Editing Technology Boost Its Applications in Livestock.

Viotti Perisse I, Fan Z, Singina G, White K, Polejaeva I Front Genet. 2021; 11:614688.

PMID: 33603767 PMC: 7885404. DOI: 10.3389/fgene.2020.614688.

SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases.

Mustafa M, Makhawi A J Clin Microbiol. 2020; 59(3).

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