Arabidopsis MicroRNA Expression Regulation in a Wide Range of Abiotic Stress Responses

Overview

Journal Front Plant Sci

Date 2015 Jun 20

PMID 26089831

Citations 85

Authors

Maria Barciszewska-Pacak

Kaja Milanowska

Katarzyna Knop

Dawid Bielewicz

Przemyslaw Nuc

Patrycja Plewka

Andrzej M Pacak

Franck Vazquez

Wojciech Karlowski

Artur Jarmolowski

Zofia Szweykowska-Kulinska

Affiliations

Soon will be listed here.

Abstract

Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess, and sulfur deficiency. A home-built RT-qPCR mirEX platform for the amplification of 289 Arabidopsis microRNA transcripts was used to study their response to abiotic stresses. Small RNA sequencing, Northern hybridization, and TaqMan® microRNA assays were performed to study the abundance of mature microRNAs. A broad response on the level of primary miRNAs (pri-miRNAs) was observed. However, stress response at the level of mature microRNAs was rather confined. The data presented show that in most instances, the level of a particular mature miRNA could not be predicted based on the level of its pri-miRNA. This points to an essential role of posttranscriptional regulation of microRNA expression. New Arabidopsis microRNAs responsive to abiotic stresses were discovered. Four microRNAs: miR319a/b, miR319b.2, and miR400 have been found to be responsive to several abiotic stresses and thus can be regarded as general stress-responsive microRNA species.

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