Silencing of Sly-miR171d Increased the Expression of and Enhanced Postharvest Chilling Tolerance of Tomato Fruit

Overview

Journal Front Plant Sci

Date 2022 Sep 26

PMID 36161008

Authors

Zengting Xing

Taishan Huang

Keyan Zhao

Lanhuan Meng

Hongmiao Song

Zhengke Zhang

Xiangbin Xu

Songbai Liu

Affiliations

Soon will be listed here.

Abstract

The role of Sly-miR171d on tomato fruit chilling injury (CI) was investigated. The results showed that silencing the endogenous Sly-miR171d effectively delayed the increase of CI and electrolyte leakage (EL) in tomato fruit, and maintained fruit firmness and quality. After low temperature storage, the expression of target gene increased in STTM-miR171d tomato fruit, the level of GA anabolism and the expression of , an important regulator of cold resistance, both increased in STTM-miR171d tomato fruit, indicated that silencing the Sly-miR171d can improve the resistance ability of postharvest tomato fruit to chilling tolerance.

Citing Articles

Mechanisms and control measures of low temperature storage-induced chilling injury to solanaceous vegetables and fruits.

Yuan Q, Jiang Y, Yang Q, Li W, Gan G, Cai L Front Plant Sci. 2024; 15:1488666.

PMID: 39588087 PMC: 11586204. DOI: 10.3389/fpls.2024.1488666.

References

Peng J, Carol P, RICHARDS D, King K, Cowling R, Murphy G . The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes Dev. 1998; 11(23):3194-205. PMC: 316750. DOI: 10.1101/gad.11.23.3194. View

Liu Y, Huang W, Xian Z, Hu N, Lin D, Ren H . Overexpression of in Tomato Enhances Tolerance to Abiotic Stresses and Influences Auxin and Gibberellin Signaling. Front Plant Sci. 2017; 8:1659. PMC: 5622987. DOI: 10.3389/fpls.2017.01659. View

Alonso-Ramirez A, Rodriguez D, Reyes D, Jimenez J, Nicolas G, Lopez-Climent M . Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiol. 2009; 150(3):1335-44. PMC: 2705047. DOI: 10.1104/pp.109.139352. View

Ding Y, Zhao J, Nie Y, Fan B, Wu S, Zhang Y . Salicylic-Acid-Induced Chilling- and Oxidative-Stress Tolerance in Relation to Gibberellin Homeostasis, C-Repeat/Dehydration-Responsive Element Binding Factor Pathway, and Antioxidant Enzyme Systems in Cold-Stored Tomato Fruit. J Agric Food Chem. 2016; 64(43):8200-8206. DOI: 10.1021/acs.jafc.6b02902. View

Habib S, Lwin Y, Li N . Down-Regulation of in Tomato Confers Abiotic Stress Tolerance. Genes (Basel). 2021; 12(5). PMC: 8143468. DOI: 10.3390/genes12050623. View

Wang Y, Feng C, Zhai Z, Peng X, Wang Y, Sun Y . The Apple Module Enhances Drought Stress Tolerance by Integrating Ascorbic Acid Metabolism. Plant Physiol. 2020; 184(1):194-211. PMC: 7479918. DOI: 10.1104/pp.20.00476. View

Huang W, Peng S, Xian Z, Lin D, Hu G, Yang L . Overexpression of a tomato miR171 target gene SlGRAS24 impacts multiple agronomical traits via regulating gibberellin and auxin homeostasis. Plant Biotechnol J. 2016; 15(4):472-488. PMC: 5362688. DOI: 10.1111/pbi.12646. View

Pino M, Skinner J, Jeknic Z, Hayes P, Soeldner A, Thomashow M . Ectopic AtCBF1 over-expression enhances freezing tolerance and induces cold acclimation-associated physiological modifications in potato. Plant Cell Environ. 2008; 31(4):393-406. DOI: 10.1111/j.1365-3040.2008.01776.x. View

Bartel D . MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116(2):281-97. DOI: 10.1016/s0092-8674(04)00045-5. View

10.

Li P, Yin F, Song L, Zheng X . Alleviation of chilling injury in tomato fruit by exogenous application of oxalic acid. Food Chem. 2016; 202:125-32. DOI: 10.1016/j.foodchem.2016.01.142. View

11.

Liu Y, Shi Y, Zhu N, Zhong S, Bouzayen M, Li Z . SlGRAS4 mediates a novel regulatory pathway promoting chilling tolerance in tomato. Plant Biotechnol J. 2020; 18(7):1620-1633. PMC: 7292549. DOI: 10.1111/pbi.13328. View

12.

Stockinger E, Gilmour S, Thomashow M . Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A. 1997; 94(3):1035-40. PMC: 19635. DOI: 10.1073/pnas.94.3.1035. View

13.

Liu Y, Wang K, Li D, Yan J, Zhang W . Enhanced Cold Tolerance and Tillering in Switchgrass (Panicum virgatum L.) by Heterologous Expression of Osa-miR393a. Plant Cell Physiol. 2017; 58(12):2226-2240. DOI: 10.1093/pcp/pcx157. View

14.

Lantzouni O, Alkofer A, Falter-Braun P, Schwechheimer C . GROWTH-REGULATING FACTORS Interact with DELLAs and Regulate Growth in Cold Stress. Plant Cell. 2020; 32(4):1018-1034. PMC: 7145461. DOI: 10.1105/tpc.19.00784. View

15.

Mekontso F, Duan W, Cisse E, Chen T, Xu X . Alleviation of Postharvest Chilling Injury of Carambola Fruit by γ-aminobutyric Acid: Physiological, Biochemical, and Structural Characterization. Front Nutr. 2021; 8:752583. PMC: 8637291. DOI: 10.3389/fnut.2021.752583. View

16.

Zhou M, Tang W . MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells. Mol Genet Genomics. 2018; 294(2):379-393. DOI: 10.1007/s00438-018-1516-4. View

17.

Bai C, Zheng Y, Watkins C, Fu A, Ma L, Gao H . Revealing the Specific Regulations of Brassinolide on Tomato Fruit Chilling Injury by Integrated Multi-Omics. Front Nutr. 2021; 8:769715. PMC: 8681340. DOI: 10.3389/fnut.2021.769715. View

18.

Achard P, Renou J, Berthome R, Harberd N, Genschik P . Plant DELLAs restrain growth and promote survival of adversity by reducing the levels of reactive oxygen species. Curr Biol. 2008; 18(9):656-60. DOI: 10.1016/j.cub.2008.04.034. View

19.

Huang J, Lin X, Zhang L, Wang X, Fan G, Chen L . MicroRNA Sequencing Revealed Adaptation to Long-Term Boron Toxicity through Modulation of Root Development by miR319 and miR171. Int J Mol Sci. 2019; 20(6). PMC: 6470687. DOI: 10.3390/ijms20061422. View

20.

Wang Z, Zhang L, Duan W, Li W, Wang Q, Li J . Melatonin maintained higher contents of unsaturated fatty acid and cell membrane structure integrity in banana peel and alleviated postharvest chilling injury. Food Chem. 2022; 397:133836. DOI: 10.1016/j.foodchem.2022.133836. View