Regulation of MiRNA Abundance by RNA Binding Protein TOUGH in Arabidopsis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2012 Jul 18

PMID 22802657

Citations 92

Authors

Guodong Ren

Meng Xie

Yongchao Dou

Shuxin Zhang

Chi Zhang

Bin Yu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.

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