Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2015 Jun 19

PMID 26085619

Citations 52

Authors

Laurent Poirel

Patrice Nordmann

Affiliations

Soon will be listed here.

Abstract

Performances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory worldwide.

Citing Articles

Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae.

Pruss A, Skierska A, Kwiatkowski P, Masiuk H, Jursa-Kulesza J, Giedrys-Kalemba S PLoS One. 2025; 20(2):e0318852.

PMID: 39919082 PMC: 11805415. DOI: 10.1371/journal.pone.0318852.

Diagnostic Test Precision of Modified Carbapenem Inactivation Method and Carbapenemase Nordmann-Poirel Test for Phenotypic Detection of Carbapenemase Production in Enterobacterales: A Systematic Review.

Suriya R V, Kv L, Feliciana J H, R A Cureus. 2024; 16(8):e67322.

PMID: 39301399 PMC: 11412648. DOI: 10.7759/cureus.67322.

Burden and Management of Multi-Drug Resistant Organism Infections in Solid Organ Transplant Recipients Across the World: A Narrative Review.

Freire M, Pouch S, Manesh A, Giannella M Transpl Int. 2024; 37:12469.

PMID: 38952482 PMC: 11215024. DOI: 10.3389/ti.2024.12469.

First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197.

De la Cadena E, Mojica M, Rojas L, Castro B, Garcia-Betancur J, Marshall S Microbiol Spectr. 2024; 12(6):e0410523.

PMID: 38700337 PMC: 11237465. DOI: 10.1128/spectrum.04105-23.

Clonal Distribution and Its Association With the Carbapenem Resistance Mechanisms of Carbapenem-Non-Susceptible Isolates From Korean Hospitals.

Kim N, Ko S, Park S, Kim S, Lee D, Kwon K Ann Lab Med. 2024; 44(5):410-417.

PMID: 38433574 PMC: 11169769. DOI: 10.3343/alm.2023.0369.

References

Poirel L, Castanheira M, Carrer A, Rodriguez C, Jones R, Smayevsky J . OXA-163, an OXA-48-related class D β-lactamase with extended activity toward expanded-spectrum cephalosporins. Antimicrob Agents Chemother. 2011; 55(6):2546-51. PMC: 3101449. DOI: 10.1128/AAC.00022-11. View

Poirel L, Walsh T, Cuvillier V, Nordmann P . Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis. 2011; 70(1):119-23. DOI: 10.1016/j.diagmicrobio.2010.12.002. View

Kempf M, Bakour S, Flaudrops C, Berrazeg M, Brunel J, Drissi M . Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PLoS One. 2012; 7(2):e31676. PMC: 3280980. DOI: 10.1371/journal.pone.0031676. View

Nordmann P, Dortet L, Poirel L . Carbapenem resistance in Enterobacteriaceae: here is the storm!. Trends Mol Med. 2012; 18(5):263-72. DOI: 10.1016/j.molmed.2012.03.003. View

Cuzon G, Naas T, Bogaerts P, Glupczynski Y, Nordmann P . Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and.... J Antimicrob Chemother. 2012; 67(8):1865-9. DOI: 10.1093/jac/dks156. View

Bernabeu S, Poirel L, Nordmann P . Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae. Diagn Microbiol Infect Dis. 2012; 74(1):88-90. DOI: 10.1016/j.diagmicrobio.2012.05.021. View

Nordmann P, Poirel L, Dortet L . Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis. 2012; 18(9):1503-7. PMC: 3437707. DOI: 10.3201/eid1809.120355. View

Osterblad M, Hakanen A, Jalava J . Evaluation of the Carba NP test for carbapenemase detection. Antimicrob Agents Chemother. 2014; 58(12):7553-6. PMC: 4249567. DOI: 10.1128/AAC.02761-13. View

Rossolini G, Arena F, Pecile P, Pollini S . Update on the antibiotic resistance crisis. Curr Opin Pharmacol. 2014; 18:56-60. DOI: 10.1016/j.coph.2014.09.006. View

10.

Adler A, Hussein O, Ben-David D, Masarwa S, Navon-Venezia S, Schwaber M . Persistence of Klebsiella pneumoniae ST258 as the predominant clone of carbapenemase-producing Enterobacteriaceae in post-acute-care hospitals in Israel, 2008-13. J Antimicrob Chemother. 2014; 70(1):89-92. DOI: 10.1093/jac/dku333. View

11.

Kulkova N, Babalova M, Sokolova J, Krcmery V . First report of New Delhi metallo-β-lactamase-1-producing strains in Slovakia. Microb Drug Resist. 2014; 21(1):117-20. DOI: 10.1089/mdr.2013.0162. View

12.

Tai A, Sidjabat H, Kidd T, Whiley D, Paterson D, Bell S . Evaluation of phenotypic screening tests for carbapenemase production in Pseudomonas aeruginosa from patients with cystic fibrosis. J Microbiol Methods. 2015; 111:105-7. DOI: 10.1016/j.mimet.2015.02.006. View

13.

Poirel L, Weldhagen G, Naas T, De Champs C, Dove M, Nordmann P . GES-2, a class A beta-lactamase from Pseudomonas aeruginosa with increased hydrolysis of imipenem. Antimicrob Agents Chemother. 2001; 45(9):2598-603. PMC: 90698. DOI: 10.1128/AAC.45.9.2598-2603.2001. View

14.

Bonnet R, Marchandin H, Chanal C, Sirot D, Labia R, De Champs C . Chromosome-encoded class D beta-lactamase OXA-23 in Proteus mirabilis. Antimicrob Agents Chemother. 2002; 46(6):2004-6. PMC: 127228. DOI: 10.1128/AAC.46.6.2004-2006.2002. View

15.

Woodford N, Ellington M, Coelho J, Turton J, Ward M, Brown S . Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents. 2006; 27(4):351-3. DOI: 10.1016/j.ijantimicag.2006.01.004. View

16.

Mugnier P, Poirel L, Naas T, Nordmann P . Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii. Emerg Infect Dis. 2009; 16(1):35-40. PMC: 2874364. DOI: 10.3201/eid1601.090852. View

17.

Dortet L, Poirel L, Nordmann P . Rapid detection of carbapenemase-producing Pseudomonas spp. J Clin Microbiol. 2012; 50(11):3773-6. PMC: 3486216. DOI: 10.1128/JCM.01597-12. View

18.

Dahyot S, Broutin I, De Champs C, Guillon H, Mammeri H . Contribution of asparagine 346 residue to the carbapenemase activity of CMY-2 β-lactamase. FEMS Microbiol Lett. 2013; 345(2):147-53. DOI: 10.1111/1574-6968.12199. View

19.

Lee W, Chung H, Lee Y, Yong D, Jeong S, Lee K . Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas.... Diagn Microbiol Infect Dis. 2013; 77(3):227-30. DOI: 10.1016/j.diagmicrobio.2013.07.005. View

20.

Dortet L, Brechard L, Cuzon G, Poirel L, Nordmann P . Strategy for rapid detection of carbapenemase-producing Enterobacteriaceae. Antimicrob Agents Chemother. 2014; 58(4):2441-5. PMC: 4023735. DOI: 10.1128/AAC.01239-13. View