Retro-translocation of Mitochondrial Intermembrane Space Proteins

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2015 Jun 10

PMID 26056291

Citations 48

Authors

Piotr Bragoszewski

Michal Wasilewski

Paulina Sakowska

Agnieszka Gornicka

Lena Bottinger

Jian Qiu

Nils Wiedemann

Agnieszka Chacinska

Affiliations

Soon will be listed here.

Abstract

The content of mitochondrial proteome is maintained through two highly dynamic processes, the influx of newly synthesized proteins from the cytosol and the protein degradation. Mitochondrial proteins are targeted to the intermembrane space by the mitochondrial intermembrane space assembly pathway that couples their import and oxidative folding. The folding trap was proposed to be a driving mechanism for the mitochondrial accumulation of these proteins. Whether the reverse movement of unfolded proteins to the cytosol occurs across the intact outer membrane is unknown. We found that reduced, conformationally destabilized proteins are released from mitochondria in a size-limited manner. We identified the general import pore protein Tom40 as an escape gate. We propose that the mitochondrial proteome is not only regulated by the import and degradation of proteins but also by their retro-translocation to the external cytosolic location. Thus, protein release is a mechanism that contributes to the mitochondrial proteome surveillance.

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