Epigenetic Memory Gained by Priming with Osteogenic Induction Medium Improves Osteogenesis and Other Properties of Mesenchymal Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2015 Jun 9

PMID 26053250

Citations 28

Authors

Yunfeng Rui

Liangliang Xu

Rui Chen

Ting Zhang

Sien Lin

Yonghui Hou

Yang Liu

Fanbiao Meng

Zhenqing Liu

Ming Ni

Kam Sze Tsang

Fuyuan Yang

Chen Wang

Hsiao Chang Chan

Xiaohua Jiang

Gang Li

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells (MSCs) are highly plastic cells that are able to transdifferentiate or dedifferentiate under appropriate conditions. In the present study, we reported here that after in vitro induction of osteogenic differentiation, MSCs could be reverted to a primitive stem cell population (dedifferentiated osteogenic MSCs, De-Os-MSCs) with improved cell survival, colony formation, osteogenic potential, migratory capacity and increased expression of Nanog, Oct4 and Sox2. Most importantly, our results showed great superiority of the De-Os-MSCs over untreated MSCs in ectopic bone formation in vivo. Furthermore, Nanog-knockdown in MSCs could reverse these enhanced properties in De-Os-MSCs in vitro, indicating a central role of Nanog in the transcriptional network. In addition, epigenetic regulations including DNA methylation and histone modifications may play important roles in regulating the de-osteogenic differentiation process. And we found decreased methylation and promoter accrual of activating histone marks, such as H3K4me3 and H4ac on both Nanog and Oct4 gene promoters. Taken together, our study demonstrated that epigenetic memory in De-Os-MSCs gained by priming with osteogenic induction medium favored their differentiation along osteoblastic lineage with improved cell survival and migratory abilities, which may have application potential in enhancing their regenerative capacity in mammals.

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