Nanog, Stat-3, and Sox-5 Involvement in Human Fetal Temporomandibular Joint Late Development

Overview

Journal J Oral Biol Craniofac Res

Publisher Elsevier

Date 2023 Aug 28

PMID 37637855

Authors

Tacia Catharine Pagni

Juliana Malta da Cunha

Daniel Martinez Saez

Adriana da Costa-Neves

Irina Kerkis

Marcelo Cavenaghi Pereira da Silva

Affiliations

Soon will be listed here.

Abstract

Background And Aim: The temporomandibular joint (TMJ) is a synovial joint that allows the complex movements essential for life. It connects the jawbone to the skull, working as a sliding hinge. Moreover, pluripotent stem cells are a source of precursors and tissue-specific cells in developing organisms, however, their biodistribution in developing fetal tissues is weakly studied. The aim of our study was analyse immunohistochemical expression of Nanog, Oct-4, Sox-2 and Stat-3 and Sox-5, in TMJ tissue samples from human fetuses aged between the 12th and 20th weeks of intrauterine life.

Materials And Methods: We fixed and processed TMJ tissue samples from human fetuses, histological sections and immunohistochemical procedures were carried out.

Results: TMJ histological studies examination did not reveal any difference in the tissue organization between the samples in the studied periods. Immunohistochemical analysis demonstrated that Oct-4 and Sox-2 lack their expression in TMJ. In contrast, Nanog was expressed in nucleous of proliferative layer of mandibular condyle, Stat-3 was expressed in nuclear cells of articular disc, Stat-3 and Sox-5 showed positive nuclear and cytoplasmic immunostaining in codrocyte layers and in ossification areas.

Conclusions: Nanog acts in maintanence of pluripotency, Stat-3 in articular disc acts as a transcriptional factor. Stat-3 and Sox-2 act in chondrocyte and osteoblast diferentiation. Distribution of the cells, which express Nanog, Stat-3, and Sox-5 in TMJ tissue during fetal development, can help further understand its physiology, pathology, and repairing capacities.

Citing Articles

Effects of botulinum toxin type A in patients with painful temporomandibular joint disorders: a systematic review and meta-analysis.

Zhu M, Huang Z, Wang Y, Qin J, Fan M Ann Med Surg (Lond). 2024; 86(7):4112-4122.

PMID: 38989167 PMC: 11230827. DOI: 10.1097/MS9.0000000000002183.

References

Lee J, Im G . SOX trio-co-transduced adipose stem cells in fibrin gel to enhance cartilage repair and delay the progression of osteoarthritis in the rat. Biomaterials. 2011; 33(7):2016-24. DOI: 10.1016/j.biomaterials.2011.11.050. View

Loh Y, Ng J, Ng H . Molecular framework underlying pluripotency. Cell Cycle. 2008; 7(7):885-91. DOI: 10.4161/cc.7.7.5636. View

Shen M, Luo Y, Niu Y, Chen L, Yuan X, Goltzman D . 1,25(OH)2D deficiency induces temporomandibular joint osteoarthritis via secretion of senescence-associated inflammatory cytokines. Bone. 2013; 55(2):400-9. DOI: 10.1016/j.bone.2013.04.015. View

Huang C, Hu F, Yu C, Tsai L, Lee T, Chou M . Concurrent expression of Oct4 and Nanog maintains mesenchymal stem-like property of human dental pulp cells. Int J Mol Sci. 2014; 15(10):18623-39. PMC: 4227236. DOI: 10.3390/ijms151018623. View

Lefebvre V, Smits P . Transcriptional control of chondrocyte fate and differentiation. Birth Defects Res C Embryo Today. 2005; 75(3):200-12. DOI: 10.1002/bdrc.20048. View

Vilanova L, Goncalves T, Meirelles L, Garcia R . Hormonal fluctuations intensify temporomandibular disorder pain without impairing masticatory function. Int J Prosthodont. 2015; 28(1):72-4. DOI: 10.11607/ijp.4040. View

Cimica V, Chen H, Iyer J, Reich N . Dynamics of the STAT3 transcription factor: nuclear import dependent on Ran and importin-β1. PLoS One. 2011; 6(5):e20188. PMC: 3098288. DOI: 10.1371/journal.pone.0020188. View

Shibukawa Y, Young B, Wu C, Yamada S, Long F, Pacifici M . Temporomandibular joint formation and condyle growth require Indian hedgehog signaling. Dev Dyn. 2006; 236(2):426-34. DOI: 10.1002/dvdy.21036. View

Lefebvre V . The SoxD transcription factors--Sox5, Sox6, and Sox13--are key cell fate modulators. Int J Biochem Cell Biol. 2009; 42(3):429-32. PMC: 2826538. DOI: 10.1016/j.biocel.2009.07.016. View

10.

de Farias J, Melo S, Bento P, Oliveira L, Campos P, de Melo D . Correlation between temporomandibular joint morphology and disc displacement by MRI. Dentomaxillofac Radiol. 2015; 44(7):20150023. PMC: 4628409. DOI: 10.1259/dmfr.20150023. View

11.

Zhang Y, Wang D, Xu J, Wang Y, Ma F, Li Z . Stat3 activation is critical for pluripotency maintenance. J Cell Physiol. 2018; 234(2):1044-1051. DOI: 10.1002/jcp.27241. View

12.

Yuan W, Fan Y, Cui M, Luo T, Wang Y, Shu Z . SOX5 Regulates Cell Proliferation, Apoptosis, Migration and Invasion in KSHV-Infected Cells. Virol Sin. 2020; 36(3):449-457. PMC: 8257826. DOI: 10.1007/s12250-020-00313-3. View

13.

Fan Y, Cui C, Li P, Bi R, Lyu P, Li Y . Fibrocartilage Stem Cells in the Temporomandibular Joint: Insights From Animal and Human Studies. Front Cell Dev Biol. 2021; 9:665995. PMC: 8111285. DOI: 10.3389/fcell.2021.665995. View

14.

Merida-Velasco J, Rodriguez-Vazquez J, Merida-Velasco J, Sanchez-Montesinos I, Espin-Ferra J, Jimenez-Collado J . Development of the human temporomandibular joint. Anat Rec. 1999; 255(1):20-33. DOI: 10.1002/(SICI)1097-0185(19990501)255:1<20::AID-AR4>3.0.CO;2-N. View

15.

Mikulenkova E, Neradil J, Vymazal O, Skoda J, Veselska R . NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation. Cells. 2020; 9(3). PMC: 7140602. DOI: 10.3390/cells9030692. View

16.

Ruscitto A, Scarpa V, Morel M, Pylawka S, Shawber C, Embree M . Notch Regulates Fibrocartilage Stem Cell Fate and Is Upregulated in Inflammatory TMJ Arthritis. J Dent Res. 2020; 99(10):1174-1181. PMC: 7443994. DOI: 10.1177/0022034520924656. View

17.

Thiel G . How Sox2 maintains neural stem cell identity. Biochem J. 2013; 450(3):e1-2. DOI: 10.1042/BJ20130176. View

18.

Pierantozzi E, Gava B, Manini I, Roviello F, Marotta G, Chiavarelli M . Pluripotency regulators in human mesenchymal stem cells: expression of NANOG but not of OCT-4 and SOX-2. Stem Cells Dev. 2010; 20(5):915-23. DOI: 10.1089/scd.2010.0353. View

19.

Lefebvre V . Roles and regulation of SOX transcription factors in skeletogenesis. Curr Top Dev Biol. 2019; 133:171-193. PMC: 6955022. DOI: 10.1016/bs.ctdb.2019.01.007. View

20.

Rui Y, Xu L, Chen R, Zhang T, Lin S, Hou Y . Epigenetic memory gained by priming with osteogenic induction medium improves osteogenesis and other properties of mesenchymal stem cells. Sci Rep. 2015; 5:11056. PMC: 4459169. DOI: 10.1038/srep11056. View