Evaluating the Antimicrobial Activity of Nisin, Lysozyme and Ethylenediaminetetraacetate Incorporated in Starch Based Active Food Packaging Film

Overview

Journal J Food Sci Technol

Specialty Nutritional Sciences

Date 2015 Jun 2

PMID 26028732

Citations 9

Authors

Sugandha Bhatia

Anoop Bharti

Affiliations

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Abstract

The pleothera of micro organisms obtained from contaminated food cultured in a starch broth was effectively tested against antibacterial agents, i.e. nisin, lysozyme and chelating agent EDTA. A variety of combination treatments of these antimicrobial agents and their incorporation in Starch based active packaging film according to their permissibility standards was done. 4 variables of Nisin concentration (ranging from 0 to 750 IU/ml), 3 variables of lysozyme concentration (ranging from 0 to 500 IU/ml) and 3 variables of EDTA concentration from (0 to 20 μM) were chosen. Bacterial inhibition by combination of different levels of different factors without antimicrobial films was evaluated using a liquid incubation method. The samples were assayed for turbidity at interval of 2, 4 and 24 h to check effectiveness of combined effects of antimicrobial agents which proved a transitory bactericidal effect for short incubation times. Zone of Inhibition was observed in the antimicrobial films prepared by agar diffusion method. Statistical analysis of experimental data for their antimicrobial spectrum was carried out by multi regression analysis and ANOVA using Design-Expert software to plot the final equation in terms of coded factors as antimicrobial agents. The experimental data indicated that the model was highly significant. Results were also evaluated graphically using response surface showing interactions between two factors, keeping other factor fixed at values at the center of domain. Synergy was also determined among antibacterial agents using the fractional inhibitory concentration (FIC) index which was observed to be 0.56 supporting the hypothesis that nisin and EDTA function as partial synergistically. The presented work aimed to screen in quick fashion the combinatorial effect of three antimicrobial agents and evaluating their efficacy in anti microbial film development.

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References

Winkowski K, Ludescher R, Montville T . Physiochemical characterization of the nisin-membrane interaction with liposomes derived from Listeria monocytogenes. Appl Environ Microbiol. 1996; 62(2):323-7. PMC: 167801. DOI: 10.1128/aem.62.2.323-327.1996. View

BERENBAUM M . Criteria for analyzing interactions between biologically active agents. Adv Cancer Res. 1981; 35:269-335. DOI: 10.1016/s0065-230x(08)60912-4. View

Hotchkiss J . Food-packaging interactions influencing quality and safety. Food Addit Contam. 1997; 14(6-7):601-7. DOI: 10.1080/02652039709374572. View

Payne K, Oliver S, Davidson P . Comparison of EDTA and Apo-Lactoferrin with Lysozyme on the Growth of Foodborne Pathogenic and Spoilage Bacteria. J Food Prot. 2019; 57(1):62-65. DOI: 10.4315/0362-028X-57.1.62. View

Montville T, Chen Y . Mechanistic action of pediocin and nisin: recent progress and unresolved questions. Appl Microbiol Biotechnol. 1999; 50(5):511-9. DOI: 10.1007/s002530051328. View

Chi-Zhang Y, Yam K, Chikindas M . Effective control of Listeria monocytogenes by combination of nisin formulated and slowly released into a broth system. Int J Food Microbiol. 2003; 90(1):15-22. DOI: 10.1016/s0168-1605(03)00168-5. View

Monk J, Beuchat L, Hathcox A . Inhibitory effects of sucrose monolaurate, alone and in combination with organic acids, on Listeria monocytogenes and Staphylococcus aureus. J Appl Bacteriol. 1996; 81(1):7-18. DOI: 10.1111/j.1365-2672.1996.tb03276.x. View

Hughey V, Johnson E . Antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease. Appl Environ Microbiol. 1987; 53(9):2165-70. PMC: 204075. DOI: 10.1128/aem.53.9.2165-2170.1987. View

Boussouel N, Mathieu F, Benoit V, Linder M, Revol-Junelles A, Milliere J . Response surface methodology, an approach to predict the effects of a lactoperoxidase system, Nisin, alone or in combination, on Listeria monocytogenes in skim milk. J Appl Microbiol. 1999; 86(4):642-52. DOI: 10.1046/j.1365-2672.1999.00707.x. View

10.

Mastromatteo M, Lucera A, Sinigaglia M, Corbo M . Use of lysozyme, nisin, and EDTA combined treatments for maintaining quality of packed ostrich patties. J Food Sci. 2010; 75(3):M178-86. DOI: 10.1111/j.1750-3841.2010.01556.x. View

11.

Padgett T, Han I, Dawson P . Incorporation of food-grade antimicrobial compounds into biodegradable packaging films. J Food Prot. 1998; 61(10):1330-5. DOI: 10.4315/0362-028x-61.10.1330. View

12.

Harris L, Fleming H, Klaenhammer T . Sensitivity and Resistance of Listeria monocytogenes ATCC 19115, Scott A, and UAL500 to Nisin. J Food Prot. 2019; 54(11):836-840. DOI: 10.4315/0362-028X-54.11.836. View

13.

Bruno M, Kaiser A, Montville T . Depletion of proton motive force by nisin in Listeria monocytogenes cells. Appl Environ Microbiol. 1992; 58(7):2255-9. PMC: 195764. DOI: 10.1128/aem.58.7.2255-2259.1992. View

14.

Murdock C, Cleveland J, Matthews K, Chikindas M . The synergistic effect of nisin and lactoferrin on the inhibition of Listeria monocytogenes and Escherichia coli O157:H7. Lett Appl Microbiol. 2007; 44(3):255-61. DOI: 10.1111/j.1472-765X.2006.02076.x. View

15.

Okereke A, Montville T . Nisin dissipates the proton motive force of the obligate anaerobe Clostridium sporogenes PA 3679. Appl Environ Microbiol. 1992; 58(8):2463-7. PMC: 195804. DOI: 10.1128/aem.58.8.2463-2467.1992. View

16.

Gill A, Holley R . Interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, nisin and EDTA in the presence of nitrite and sodium chloride at 24 degrees C. Int J Food Microbiol. 2002; 80(3):251-9. DOI: 10.1016/s0168-1605(02)00171-x. View

17.

Hurst A . Function of nisin and nisin-like basic proteins in the growth cycle of streptococcus lactis. Nature. 1967; 214(5094):1232-4. DOI: 10.1038/2141232a0. View

18.

Shively J, HARTSELL S . BACTERIOLYSIS OF THE PSEUDOMONADS. I. AGENTS POTENTIATING LYSIS. Can J Microbiol. 1964; 10:905-9. DOI: 10.1139/m64-117. View

19.

Parente E, Giglio M, Ricciardi A, Clementi F . The combined effect of nisin, leucocin F10, pH, NaCl and EDTA on the survival of Listeria monocytogenes in broth. Int J Food Microbiol. 1998; 40(1-2):65-75. DOI: 10.1016/s0168-1605(98)00021-x. View

20.

Vessoni P, Moraes D, Fajardo D . The effect of nisin on growth kinetics from activated Bacillus cereus spores in cooked rice and in milk. J Food Prot. 2002; 65(2):419-22. View