Applications of Nisin and EDTA in Food Packaging for Improving Fabricated Chitosan-Polylactate Plastic Film Performance and Fish Fillet Preservation

Overview

Journal Membranes (Basel)

Date 2021 Nov 27

PMID 34832081

Citations 7

Authors

Shun-Hsien Chang

Ying-Ju Chen

Hsiang-Jung Tseng

Hsin-I Hsiao

Huey-Jine Chai

Kuo-Chung Shang

Chorng-Liang Pan

Guo-Jane Tsai

Affiliations

Soon will be listed here.

Abstract

This study aimed to increase the antibacterial activity of chitosan-polylactic acid (PLA) composite film by adding nisin and ethylenediaminetetraacetic acid (EDTA). We evaluated the mechanical, physicochemical, and antibacterial properties of various PLA composite films, as well as the enhancement effect of PLA composite films with EDTA + nisin on the preservation of grouper fillets. Films of PLA alone, PLA plus chitosan (C5), PLA plus nisin + EDTA (EN2), and PLA plus chitosan plus nisin + EDTA (C5EN1 and C5EN2) were prepared. The addition of EDTA + nisin to the chitosan-PLA matrix significantly improved the antibacterial activity of the PLA composite film, with C5EN1 and C5EN2 films showing the highest antibacterial activity among the five films. Compared with the fish samples covered by C5, the counts of several microbial categories (i.e., mesophilic bacteria, psychrotrophic bacteria, coliforms, , , and ) and total volatile basic nitrogen content in fish were significantly reduced in the samples covered by C5EN1. In addition, the counts of samples covered by C5EN1 or C5 were significantly lower compared to the uncovered and PLA film-covered samples.

Citing Articles

Harnessing the Power of Bacteriocins: A Comprehensive Review on Sources, Mechanisms, and Applications in Food Preservation and Safety.

Singh B, Kumar N, Yadav A, Rohan , Bhandari K Curr Microbiol. 2025; 82(4):174.

PMID: 40053112 DOI: 10.1007/s00284-025-04155-8.

New advances in biological preservation technology for aquatic products.

Nie X, Zuo Z, Zhang R, Luo S, Chi Y, Yuan X NPJ Sci Food. 2025; 9(1):15.

PMID: 39900935 PMC: 11790869. DOI: 10.1038/s41538-025-00372-4.

Edible Coatings for Fish Preservation: Literature Data on Storage Temperature, Product Requirements, Antioxidant Activity, and Coating Performance-A Review.

Kurek M, Pisonic P, Scetar M, Janci T, canak I, Vidacek Filipec S Antioxidants (Basel). 2024; 13(11).

PMID: 39594558 PMC: 11591116. DOI: 10.3390/antiox13111417.

Evaluation of PLA-Based Composite Films Filled with Cu(OH)NO Nanoparticles as an Active Material for the Food Industry: Biocidal Properties and Environmental Sustainability.

Santos X, Dominguez G, Rodriguez J, Pozuelo J, Hernandez M, Martin O Polymers (Basel). 2024; 16(13).

PMID: 39000628 PMC: 11243838. DOI: 10.3390/polym16131772.

Synergistic effects of EDTA and lysozyme on the properties of hydroxypropyl starch nano antibacterial films.

Shao X, Sun H, Wang X, Zhou R Curr Res Food Sci. 2024; 8:100657.

PMID: 38204880 PMC: 10777376. DOI: 10.1016/j.crfs.2023.100657.

References

Giannakourou M, Tsironi T . Application of Processing and Packaging Hurdles for Fresh-Cut Fruits and Vegetables Preservation. Foods. 2021; 10(4). PMC: 8068883. DOI: 10.3390/foods10040830. View

Hoffman K, Han I, Dawson P . Antimicrobial effects of corn zein films impregnated with nisin, lauric acid, and EDTA. J Food Prot. 2001; 64(6):885-9. DOI: 10.4315/0362-028x-64.6.885. View

Lei J, Sun L, Huang S, Zhu C, Li P, He J . The antimicrobial peptides and their potential clinical applications. Am J Transl Res. 2019; 11(7):3919-3931. PMC: 6684887. View

Bhatia S, Bharti A . Evaluating the antimicrobial activity of Nisin, Lysozyme and Ethylenediaminetetraacetate incorporated in starch based active food packaging film. J Food Sci Technol. 2015; 52(6):3504-12. PMC: 4444913. DOI: 10.1007/s13197-014-1414-7. View

Yu Z, Li B, Chu J, Zhang P . Silica in situ enhanced PVA/chitosan biodegradable films for food packages. Carbohydr Polym. 2018; 184:214-220. DOI: 10.1016/j.carbpol.2017.12.043. View

Remya S, Mohan C, Bindu J, Sivaraman G, Venkateshwarlu G, Ravishankar C . Effect of chitosan based active packaging film on the keeping quality of chilled stored barracuda fish. J Food Sci Technol. 2016; 53(1):685-93. PMC: 4711446. DOI: 10.1007/s13197-015-2018-6. View

Grande R, Carvalho A . Compatible ternary blends of chitosan/poly(vinyl alcohol)/poly(lactic acid) produced by oil-in-water emulsion processing. Biomacromolecules. 2011; 12(4):907-14. DOI: 10.1021/bm101227q. View

Agriopoulou S, Stamatelopoulou E, Varzakas T . Advances in Occurrence, Importance, and Mycotoxin Control Strategies: Prevention and Detoxification in Foods. Foods. 2020; 9(2). PMC: 7074356. DOI: 10.3390/foods9020137. View

Hui G, Liu W, Feng H, Li J, Gao Y . Effects of chitosan combined with nisin treatment on storage quality of large yellow croaker (Pseudosciaena crocea). Food Chem. 2016; 203:276-282. DOI: 10.1016/j.foodchem.2016.01.122. View

10.

Tovar L, Salafranca J, Sanchez C, Nerin C . Migration studies to assess the safety in use of a new antioxidant active packaging. J Agric Food Chem. 2005; 53(13):5270-5. DOI: 10.1021/jf050076k. View

11.

Belfiore C, Castellano P, Vignolo G . Reduction of Escherichia coli population following treatment with bacteriocins from lactic acid bacteria and chelators. Food Microbiol. 2006; 24(3):223-9. DOI: 10.1016/j.fm.2006.05.006. View

12.

Cole J, Nizet V . Bacterial Evasion of Host Antimicrobial Peptide Defenses. Microbiol Spectr. 2016; 4(1). PMC: 4804471. DOI: 10.1128/microbiolspec.VMBF-0006-2015. View

13.

Vasile C . Polymeric Nanocomposites and Nanocoatings for Food Packaging: A Review. Materials (Basel). 2018; 11(10). PMC: 6213312. DOI: 10.3390/ma11101834. View

14.

Zimet P, Mombru A, Mombru D, Castro A, Villanueva J, Pardo H . Physico-chemical and antilisterial properties of nisin-incorporated chitosan/carboxymethyl chitosan films. Carbohydr Polym. 2019; 219:334-343. DOI: 10.1016/j.carbpol.2019.05.013. View

15.

Ukuku D, Fett W . Effect of nisin in combination with EDTA, sodium lactate, and potassium sorbate for reducing Salmonella on whole and fresh-cut cantaloupet. J Food Prot. 2004; 67(10):2143-50. DOI: 10.4315/0362-028x-67.10.2143. View

16.

Martau G, Mihai M, Vodnar D . The Use of Chitosan, Alginate, and Pectin in the Biomedical and Food Sector-Biocompatibility, Bioadhesiveness, and Biodegradability. Polymers (Basel). 2019; 11(11). PMC: 6918388. DOI: 10.3390/polym11111837. View

17.

Castro-Rosas J, Ferreira-Grosso C, Gomez-Aldapa C, Rangel-Vargas E, Rodriguez-Marin M, Guzman-Ortiz F . Recent advances in microencapsulation of natural sources of antimicrobial compounds used in food - A review. Food Res Int. 2017; 102:575-587. DOI: 10.1016/j.foodres.2017.09.054. View

18.

Salmaso S, Elvassore N, Bertucco A, Lante A, Caliceti P . Nisin-loaded poly-L-lactide nano-particles produced by CO2 anti-solvent precipitation for sustained antimicrobial activity. Int J Pharm. 2004; 287(1-2):163-73. DOI: 10.1016/j.ijpharm.2004.09.003. View

19.

Chang S, Chen Y, Tseng H, Hsiao H, Chai H, Shang K . Antibacterial Activity of Chitosan-Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet. Polymers (Basel). 2021; 13(5). PMC: 7956300. DOI: 10.3390/polym13050696. View

20.

Kim S, Becattini S, Moody T, Shliaha P, Littmann E, Seok R . Microbiota-derived lantibiotic restores resistance against vancomycin-resistant Enterococcus. Nature. 2019; 572(7771):665-669. PMC: 6717508. DOI: 10.1038/s41586-019-1501-z. View