Generation and Evaluation of a Recombinant Genotype VII Newcastle Disease Virus Expressing VP3 Protein of Goose Parvovirus As a Bivalent Vaccine in Goslings

Overview

Journal Virus Res

Specialty Microbiology

Date 2015 Apr 18

PMID 25882914

Citations 17

Authors

Jianzhong Wang

Yanlong Cong

Renfu Yin

Na Feng

Songtao Yang

Xianzhu Xia

Yueqiang Xiao

Wenxiu Wang

Xiufan Liu

Shunlin Hu

Chan Ding

Shengqing Yu

Chunfeng Wang

Zhuang Ding

Affiliations

Soon will be listed here.

Abstract

Newcastle disease virus (NDV) and Goose parvovirus (GPV) are considered to be two of the most important and widespread viruses infecting geese. In this study, we generated a recombinant rmNA-VP3, expressing GPV VP3 using a modified goose-origin NDV NA-1 by changing the multi-basic cleavage site motif RRQKR↓F of the F protein to the dibasic motif GRQGR↓L as that of the avirulent strain LaSota as a vaccine vector. Expression of the VP3 protein in rmNA-VP3 infected cells was detected by immunofluorescence and Western blot assay. The genetic stability was examined by serially passaging 10 times in 10-day-old embryonated SPF chicken eggs. Goslings were inoculated with rmNA-VP3 showed no apparent signs of disease and developed a strong GPV and NDV neutralizing antibodies response. This is the first study demonstrating that recombinant NDV has the potential to serve as bivalent live vaccine against Goose parvovirus and Newcastle disease virus infection in birds.

Citing Articles

Development of a Novel Chimeric ND-GP cVLPs Vaccine for the Prevention of Goose-Derived Newcastle Disease and Gosling Plague.

Li J, Ding J, Guo C, Xu X, Shan C, Qian J Microorganisms. 2024; 12(11).

PMID: 39597655 PMC: 11596917. DOI: 10.3390/microorganisms12112266.

The Application of Newcastle Disease Virus (NDV): Vaccine Vectors and Tumor Therapy.

Yang H, Tian J, Zhao J, Zhao Y, Zhang G Viruses. 2024; 16(6).

PMID: 38932177 PMC: 11209082. DOI: 10.3390/v16060886.

Negative-Strand RNA Virus-Vectored Vaccines.

Murr M, Mettenleiter T Methods Mol Biol. 2024; 2786:51-87.

PMID: 38814390 DOI: 10.1007/978-1-0716-3770-8_3.

Development and Scalable Production of Newcastle Disease Virus-Vectored Vaccines for Human and Veterinary Use.

Fulber J, Kamen A Viruses. 2022; 14(5).

PMID: 35632717 PMC: 9143368. DOI: 10.3390/v14050975.

Avian Orthoavulavirus Type-1 as Vaccine Vector against Respiratory Viral Pathogens in Animal and Human.

Vilela J, Rohaim M, Munir M Vaccines (Basel). 2022; 10(2).

PMID: 35214716 PMC: 8876055. DOI: 10.3390/vaccines10020259.

References

Huang Z, Elankumaran S, Panda A, Samal S . Recombinant Newcastle disease virus as a vaccine vector. Poult Sci. 2003; 82(6):899-906. DOI: 10.1093/ps/82.6.899. View

Ge J, Wang X, Tian M, Wen Z, Feng Q, Qi X . Novel in-ovo chimeric recombinant Newcastle disease vaccine protects against both Newcastle disease and infectious bursal disease. Vaccine. 2014; 32(13):1514-21. DOI: 10.1016/j.vaccine.2014.01.020. View

Jansson D, Feinstein R, Kardi V, Mato T, Palya V . Epidemiologic investigation of an outbreak of goose parvovirus infection in Sweden. Avian Dis. 2007; 51(2):609-13. DOI: 10.1637/0005-2086(2007)51[609:EIOAOO]2.0.CO;2. View

Gough R, Spackman D . Studies with a duck embryo adapted goose parvovirus vaccine. Avian Pathol. 1982; 11(3):503-10. DOI: 10.1080/03079458208436121. View

Alexander D . Newcastle disease and other avian paramyxoviruses. Rev Sci Tech. 2000; 19(2):443-62. DOI: 10.20506/rst.19.2.1231. View

Kisary J . Immunological aspects of Derzsy's disease in goslings. Avian Pathol. 1977; 6(4):327-34. DOI: 10.1080/03079457708418242. View

Perozo F, Marcano R, Afonso C . Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. J Clin Microbiol. 2012; 50(4):1204-8. PMC: 3318500. DOI: 10.1128/JCM.06506-11. View

Wang J, Wang C, Feng N, Wang H, Zheng X, Yang S . Development of a reverse genetics system based on RNA polymerase II for Newcastle disease virus genotype VII. Virus Genes. 2014; 50(1):152-5. DOI: 10.1007/s11262-014-1137-x. View

Derzsy D . A viral disease of goslings. I. Epidemiological, clinical, pathological and aetiological studies. Acta Vet Acad Sci Hung. 1967; 17(4):443-8. View

10.

Kim S, Paldurai A, Xiao S, Collins P, Samal S . Modified Newcastle disease virus vectors expressing the H5 hemagglutinin induce enhanced protection against highly pathogenic H5N1 avian influenza virus in chickens. Vaccine. 2014; 32(35):4428-4435. PMC: 4794254. DOI: 10.1016/j.vaccine.2014.06.061. View

11.

Xu M, Chang S, Ding Z, Gao H, Wan J, Liu W . Genomic analysis of Newcastle disease virus strain NA-1 isolated from geese in China. Arch Virol. 2008; 153(7):1281-9. DOI: 10.1007/s00705-008-0115-9. View

12.

Miller P, King D, Afonso C, Suarez D . Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge. Vaccine. 2007; 25(41):7238-46. DOI: 10.1016/j.vaccine.2007.07.017. View

13.

Panda A, Huang Z, Elankumaran S, Rockemann D, Samal S . Role of fusion protein cleavage site in the virulence of Newcastle disease virus. Microb Pathog. 2003; 36(1):1-10. PMC: 7125746. DOI: 10.1016/j.micpath.2003.07.003. View

14.

DiNapoli J, Kotelkin A, Yang L, Elankumaran S, Murphy B, Samal S . Newcastle disease virus, a host range-restricted virus, as a vaccine vector for intranasal immunization against emerging pathogens. Proc Natl Acad Sci U S A. 2007; 104(23):9788-93. PMC: 1887550. DOI: 10.1073/pnas.0703584104. View

15.

Huang Z, Elankumaran S, Yunus A, Samal S . A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol. 2004; 78(18):10054-63. PMC: 514986. DOI: 10.1128/JVI.78.18.10054-10063.2004. View

16.

Le Gall-Recule G, Jestin V, Chagnaud P, Blanchard P, Jestin A . Expression of muscovy duck parvovirus capsid proteins (VP2 and VP3) in a baculovirus expression system and demonstration of immunity induced by the recombinant proteins. J Gen Virol. 1996; 77 ( Pt 9):2159-63. DOI: 10.1099/0022-1317-77-9-2159. View

17.

Takehara K, Nishio T, Hayashi Y, Kanda J, Sasaki M, Abe N . An outbreak of goose parvovirus infection in Japan. J Vet Med Sci. 1995; 57(4):777-9. DOI: 10.1292/jvms.57.777. View

18.

Hu Z, Hu S, Meng C, Wang X, Zhu J, Liu X . Generation of a genotype VII Newcastle disease virus vaccine candidate with high yield in embryonated chicken eggs. Avian Dis. 2011; 55(3):391-7. DOI: 10.1637/9633-122410-Reg.1. View

19.

Munir M, Zohari S, Berg M . Newcastle disease virus in pakistan: genetic characterization and implication in molecular diagnosis. Indian J Virol. 2013; 23(3):368-73. PMC: 3550779. DOI: 10.1007/s13337-012-0073-4. View

20.

Jinding C, Ming L, Tao R, Chaoan X . A goose-sourced paramyxovirus isolated from southern China. Avian Dis. 2005; 49(1):170-3. DOI: 10.1637/7270-090101R1. View