Immunogenicity of Recombinant NC8 Expressing Goose Parvovirus VP2 Gene in BALB/c Mice

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2016 Jul 27

PMID 27456769

Citations 7

Authors

Yu-Ying Liu

Wen-Tao Yang

Shao-Hua Shi

Ya-Jie Li

Liang Zhao

Chun-Wei Shi

Fang-Yu Zhou

Yan-Long Jiang

Jing-Tao Hu

Wei Gu

Gui-Lian Yang

Chun-Feng Wang

Affiliations

Soon will be listed here.

Abstract

Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 10 colony-forming unit/200 μL dose of the recombinant strain, and the ratios and numbers of CD11c, CD3CD4, CD3CD8, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.

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