Multi-lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Apr 8

PMID 25848763

Citations 26

Authors

Hamad Ali

Majda K Al-Yatama

Mohamed Abu-Farha

Kazem Behbehani

Ashraf Al Madhoun

Affiliations

Soon will be listed here.

Abstract

Wharton's Jelly- derived Mesenchymal stem cells (WJ-MSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal, differentiation and unique immunomodulatory properties. Although many studies have characterized various WJ-MSCs biologically, the expression profiles of the commonly used stemness markers have not yet been addressed. In this study, WJ-MSCs were isolated and characterized for stemness and surface markers expression. Flow cytometry, immunofluorescence and qRT-PCR analysis revealed predominant expression of CD29, CD44, CD73, CD90, CD105 and CD166 in WJ-MSCs, while the hematopoietic and endothelial markers were absent. Differential expression of CD 29, CD90, CD105 and CD166 following adipogenic, osteogenic and chondrogenic induction was observed. Furthermore, our results demonstrated a reduction in CD44 and CD73 expressions in response to the tri-lineage differentiation induction, suggesting that they can be used as reliable stemness markers, since their expression was associated with undifferentiated WJ-MSCs only.

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References

Jiang R, Han Z, Zhuo G, Qu X, Li X, Wang X . Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study. Front Med. 2011; 5(1):94-100. DOI: 10.1007/s11684-011-0116-z. View

Brunner M, Millon-Fremillon A, Chevalier G, Nakchbandi I, Mosher D, Block M . Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition. J Cell Biol. 2011; 194(2):307-22. PMC: 3144405. DOI: 10.1083/jcb.201007108. View

Seshareddy K, Troyer D, Weiss M . Method to isolate mesenchymal-like cells from Wharton's Jelly of umbilical cord. Methods Cell Biol. 2008; 86:101-19. DOI: 10.1016/S0091-679X(08)00006-X. View

Dmitrieva R, Minullina I, Bilibina A, Tarasova O, Anisimov S, Zaritskey A . Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: differences and similarities. Cell Cycle. 2011; 11(2):377-83. DOI: 10.4161/cc.11.2.18858. View

Sarugaser R, Hanoun L, Keating A, Stanford W, Davies J . Human mesenchymal stem cells self-renew and differentiate according to a deterministic hierarchy. PLoS One. 2009; 4(8):e6498. PMC: 2714967. DOI: 10.1371/journal.pone.0006498. View

Jarvinen L, Badri L, Wettlaufer S, Ohtsuka T, Standiford T, Toews G . Lung resident mesenchymal stem cells isolated from human lung allografts inhibit T cell proliferation via a soluble mediator. J Immunol. 2008; 181(6):4389-96. PMC: 3644960. DOI: 10.4049/jimmunol.181.6.4389. View

Khadir A, Tiss A, Abubaker J, Abu-Farha M, Al-Khairi I, Cherian P . MAP kinase phosphatase DUSP1 is overexpressed in obese humans and modulated by physical exercise. Am J Physiol Endocrinol Metab. 2014; 308(1):E71-83. DOI: 10.1152/ajpendo.00577.2013. View

Devine S, Cobbs C, Jennings M, Bartholomew A, Hoffman R . Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates. Blood. 2002; 101(8):2999-3001. DOI: 10.1182/blood-2002-06-1830. View

Martins A, Paiva A, Morgado J, Gomes A, Pais M . Quantification and immunophenotypic characterization of bone marrow and umbilical cord blood mesenchymal stem cells by multicolor flow cytometry. Transplant Proc. 2009; 41(3):943-6. DOI: 10.1016/j.transproceed.2009.01.059. View

10.

Fong C, Chak L, Biswas A, Tan J, Gauthaman K, Chan W . Human Wharton's jelly stem cells have unique transcriptome profiles compared to human embryonic stem cells and other mesenchymal stem cells. Stem Cell Rev Rep. 2010; 7(1):1-16. DOI: 10.1007/s12015-010-9166-x. View

11.

Bassi E, Aita C, Camara N . Immune regulatory properties of multipotent mesenchymal stromal cells: Where do we stand?. World J Stem Cells. 2011; 3(1):1-8. PMC: 3097934. DOI: 10.4252/wjsc.v3.i1.1. View

12.

Voronova A, Coyne E, Madhoun A, Fair J, Bosiljcic N, St-Louis C . Hedgehog signaling regulates MyoD expression and activity. J Biol Chem. 2012; 288(6):4389-404. PMC: 3567689. DOI: 10.1074/jbc.M112.400184. View

13.

Barker T, Hagood J . Getting a grip on Thy-1 signaling. Biochim Biophys Acta. 2008; 1793(5):921-3. PMC: 2701903. DOI: 10.1016/j.bbamcr.2008.10.004. View

14.

Zhang W, Ge W, Li C, You S, Liao L, Han Q . Effects of mesenchymal stem cells on differentiation, maturation, and function of human monocyte-derived dendritic cells. Stem Cells Dev. 2004; 13(3):263-71. DOI: 10.1089/154732804323099190. View

15.

Kim D, Staples M, Shinozuka K, Pantcheva P, Kang S, Borlongan C . Wharton's jelly-derived mesenchymal stem cells: phenotypic characterization and optimizing their therapeutic potential for clinical applications. Int J Mol Sci. 2013; 14(6):11692-712. PMC: 3709752. DOI: 10.3390/ijms140611692. View

16.

Hu L, Hu J, Zhao J, Liu J, Ouyang W, Yang C . Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells. Biomed Res Int. 2013; 2013:438243. PMC: 3722850. DOI: 10.1155/2013/438243. View

17.

Jeong J, Ko K, Park H, Lee J, Jang C, Jeon C . Membrane proteomic analysis of human mesenchymal stromal cells during adipogenesis. Proteomics. 2007; 7(22):4181-91. DOI: 10.1002/pmic.200700502. View

18.

Savage J, Voronova A, Mehta V, Sendi-Mukasa F, Skerjanc I . Canonical Wnt signaling regulates Foxc1/2 expression in P19 cells. Differentiation. 2009; 79(1):31-40. DOI: 10.1016/j.diff.2009.08.008. View

19.

Ode A, Kopf J, Kurtz A, Schmidt-Bleek K, Schrade P, Kolar P . CD73 and CD29 concurrently mediate the mechanically induced decrease of migratory capacity of mesenchymal stromal cells. Eur Cell Mater. 2011; 22:26-42. DOI: 10.22203/ecm.v022a03. View

20.

Jurga M, Forraz N, Basford C, Atzeni G, Trevelyan A, Habibollah S . Neurogenic properties and a clinical relevance of multipotent stem cells derived from cord blood samples stored in the biobanks. Stem Cells Dev. 2011; 21(6):923-36. DOI: 10.1089/scd.2011.0224. View