Evaluation of a Radioactive RRNA:cDNA-hybridisation Assay for the Direct Detection of Chlamydia Trachomatis in Urogenital Specimens

Overview

Journal Genitourin Med

Specialty Infectious Diseases

Date 1989 Oct 1

PMID 2583715

Citations 2

Authors

H Naher

B Niebauer

M Hartmann

J SOLTZ-SZOTS

D Petzoldt

Affiliations

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Abstract

A radioactive cDNA probe complementary to chlamydial ribosomal RNA was used to detect C trachomatis in urogenital specimens. Of 37 specimens positive with cell culture 31 were confirmed by the rRNA:cDNA hybridisation test, the sensitivity being 83.8%. The specificity of the hybridisation test was 94.4%, as 186 of 197 specimens that were negative by cell culture were also negative when assessed by the hybridisation method. Given a prevalence of 15.8% the predictive values for positive and negative results were 73.8% and 96.9%, respectively. In additional experiments the possible role of microorganisms added to the specimen collection medium was investigated. However, no indication for crosshybridisation was found; at high concentrations microorganisms interfered with the test procedure.

Citing Articles

Detection of Chlamydia trachomatis in clinical specimens by the polymerase chain reaction.

Claas H, Melchers W, de Bruijn I, de Graaf M, van Dijk W, Lindeman J Eur J Clin Microbiol Infect Dis. 1990; 9(12):864-8.

PMID: 2073896 DOI: 10.1007/BF01967500.

Detection of C trachomatis in urogenital specimens by polymerase chain reaction.

Naher H, Drzonek H, Wolf J, Knebel Doeberitz M, Petzoldt D Genitourin Med. 1991; 67(3):211-4.

PMID: 2071122 PMC: 1194674. DOI: 10.1136/sti.67.3.211.

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