Use of Nonradioactive DNA Probes for the Detection of Infectious Bacteria

Overview

Journal Clin Lab Med

Specialty Pathology

Date 1985 Sep 1

PMID 2994943

Citations 5

Authors

M Pollice

H L Yang

Affiliations

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Abstract

Application of the biotinylated DNA probe systems in the detection of Chlamydia trachomatis and penicillin-resistant Neisseria gonorrhoeae is presented. The three formats employed are the dot-blot, in-situ, and Southern hybridization.

Citing Articles

DNA probes for clinical applications. Patents and literature.

Linhardt R Appl Biochem Biotechnol. 1986; 12(3):301-10.

PMID: 3530132 DOI: 10.1007/BF02798426.

Evaluation of a radioactive rRNA:cDNA-hybridisation assay for the direct detection of Chlamydia trachomatis in urogenital specimens.

Naher H, Niebauer B, Hartmann M, SOLTZ-SZOTS J, Petzoldt D Genitourin Med. 1989; 65(5):319-22.

PMID: 2583715 PMC: 1194385. DOI: 10.1136/sti.65.5.319.

Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

Running J, Stempien M, Urdea M Antimicrob Agents Chemother. 1989; 33(10):1813-5.

PMID: 2511803 PMC: 172761. DOI: 10.1128/AAC.33.10.1813.

Detection of bacteria by hybridization of rRNA with DNA-latex and immunodetection of hybrids.

Miller C, PATTERSON W, JOHNSON P, Swartzell C, Wogoman F, Albarella J J Clin Microbiol. 1988; 26(7):1271-6.

PMID: 2457597 PMC: 266591. DOI: 10.1128/jcm.26.7.1271-1276.1988.

[Technic of nucleic acid hybridization and its significance for diagnostic problems].

Neumann R Naturwissenschaften. 1987; 74(3):125-33.

PMID: 2437460 DOI: 10.1007/BF00366520.