Cyclic GMP Balance is Critical for Malaria Parasite Transmission from the Mosquito to the Mammalian Host

Overview

Journal mBio

Publisher American Society for Microbiology

Specialty Microbiology

Date 2015 Mar 19

PMID 25784701

Citations 18

Authors

Viswanathan Lakshmanan

Matthew E Fishbaugher

Bob Morrison

Michael Baldwin

Michael Macarulay

Ashley M Vaughan

Sebastian A Mikolajczak

Stefan H I Kappe

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Transmission of malaria occurs during Anopheles mosquito vector blood meals, when Plasmodium sporozoites that have invaded the mosquito salivary glands are delivered to the mammalian host. Sporozoites display a unique form of motility that is essential for their movement across cellular host barriers and invasion of hepatocytes. While the molecular machinery powering motility and invasion is increasingly well defined, the signaling events that control these essential parasite activities have not been clearly delineated. Here, we identify a phosphodiesterase (PDEγ) in Plasmodium, a regulator of signaling through cyclic nucleotide second messengers. Reverse transcriptase PCR (RT-PCR) analysis and epitope tagging of endogenous PDEγ detected its expression in blood stages and sporozoites of Plasmodium yoelii. Deletion of PDEγ (pdeγ(-)) rendered sporozoites nonmotile, and they failed to invade the mosquito salivary glands. Consequently, PDEγ deletion completely blocked parasite transmission by mosquito bite. Strikingly, pdeγ(-) sporozoites showed dramatically elevated levels of cyclic GMP (cGMP), indicating that a perturbation in cyclic nucleotide balance is involved in the observed phenotypic defects. Transcriptome sequencing (RNA-Seq) analysis of pdeγ(-) sporozoites revealed reduced transcript abundance of genes that encode key components of the motility and invasion apparatus. Our data reveal a crucial role for PDEγ in maintaining the cyclic nucleotide balance in the malaria parasite sporozoite stage, which in turn is essential for parasite transmission from mosquito to mammal.

Importance: Malaria is a formidable threat to human health worldwide, and there is an urgent need to identify novel drug targets for this parasitic disease. The parasite is transmitted by mosquito bite, inoculating the host with infectious sporozoite stages. We show that cellular signaling by cyclic nucleotides is critical for transmission of the parasite from the mosquito vector to the mammalian host. Parasite phosphodiesterase γ is essential for maintaining cyclic nucleotide balance, and its deletion blocks transmission of sporozoites. A deeper understanding of the signaling mechanisms involved in transmission might inform the discovery of novel drugs that interrupt this essential step in the parasite life cycle.

Citing Articles

Inhibitors of malaria parasite cyclic nucleotide phosphodiesterases block asexual blood-stage development and mosquito transmission.

Gomez-Gonzalez P, Gupta A, Drought L, Patel A, Okombo J, van der Watt M Sci Adv. 2024; 10(49):eadq1383.

PMID: 39642214 PMC: 11623267. DOI: 10.1126/sciadv.adq1383.

Apicomplexan phosphodiesterases in cyclic nucleotide turnover: conservation, function, and therapeutic potential.

Moss W, Brusini L, Kuehnel R, Brochet M, Brown K mBio. 2023; 15(2):e0305623.

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Plasticity and therapeutic potential of cAMP and cGMP-specific phosphodiesterases in .

Vo K, Ruga L, Psathaki O, Franzkoch R, Distler U, Tenzer S Comput Struct Biotechnol J. 2022; 20:5775-5789.

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Guanylyl Cyclase-Alpha and the Activity of Its Appended P4-ATPase Domain Are Essential for cGMP Synthesis and Blood-Stage Egress.

Nofal S, Patel A, Blackman M, Flueck C, Baker D mBio. 2021; 12(1).

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Comparative transcriptomics and host-specific parasite gene expression profiles inform on drivers of proliferative kidney disease.

Faber M, Shaw S, Yoon S, de Paiva Alves E, Wang B, Qi Z Sci Rep. 2021; 11(1):2149.

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