Plasticity and Therapeutic Potential of CAMP and CGMP-specific Phosphodiesterases in

is a common zoonotic protozoan pathogen adapted to intracellular parasitism in many host cells of diverse organisms. Our previous work has identified 18 cyclic nucleotide phosphodiesterase (PDE) proteins encoded by the parasite genome, of which 11 are expressed during the lytic cycle of its acutely-infectious tachyzoite stage in human cells. Here, we show that ten of these enzymes are promiscuous dual-specific phosphodiesterases, hydrolyzing cAMP and cGMP. PDE1 and PDE9, with a K of 18 μM and 31 μM, respectively, are primed to hydrolyze cGMP, whereas PDE2 is highly specific to cAMP (K, 14 μM). Immuno-electron microscopy revealed various subcellular distributions of PDE1, 2, and 9, including in the inner membrane complex, apical pole, plasma membrane, cytosol, dense granule, and rhoptry, indicating spatial control of signaling within tachyzoites. Notably, despite shared apical location and dual-catalysis, PDE8 and PDE9 are fully dispensable for the lytic cycle and show no functional redundancy. In contrast, PDE1 and PDE2 are individually required for optimal growth, and their collective loss is lethal to the parasite. phenotyping of these mutants revealed the roles of PDE1 and PDE2 in proliferation, gliding motility, invasion and egress of tachyzoites. Moreover, our enzyme inhibition assays in conjunction with chemogenetic phenotyping underpin PDE1 as a target of commonly-used PDE inhibitors, BIPPO and zaprinast. Finally, we identified a retinue of PDE1 and PDE2-interacting kinases and phosphatases, possibly regulating the enzymatic activity. In conclusion, our datasets on the catalytic function, physiological relevance, subcellular localization and drug inhibition of key phosphodiesterases highlight the previously-unanticipated plasticity and therapeutic potential of cyclic nucleotide signaling in .