Assay Methods for H2S Biogenesis and Catabolism Enzymes

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2015 Mar 2

PMID 25725523

Citations 19

Authors

Ruma Banerjee

Taurai Chiku

Omer Kabil

Marouane Libiad

Nicole Motl

Pramod K Yadav

Affiliations

Soon will be listed here.

Abstract

H2S is produced from sulfur-containing amino acids, cysteine and homocysteine, or a catabolite, 3-mercaptopyruvate, by three known enzymes: cystathionine β-synthase, γ-cystathionase, and 3-mercaptopyruvate sulfurtransferase. Of these, the first two enzymes reside in the cytoplasm and comprise the transsulfuration pathway, while the third enzyme is found both in the cytoplasm and in the mitochondrion. The following mitochondrial enzymes oxidize H2S: sulfide quinone oxidoreductase, sulfur dioxygenase, rhodanese, and sulfite oxidase. The products of the sulfide oxidation pathway are thiosulfate and sulfate. Assays for enzymes involved in the production and oxidative clearance of sulfide to thiosulfate are described in this chapter.

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