Preparation of Poly(3-hydroxybutyrate-co-hydroxyvalerate) Films from Halophilic Archaea and Their Potential Use in Drug Delivery

Overview

Journal Extremophiles

Publisher Springer

Date 2015 Feb 10

PMID 25663452

Citations 10

Authors

Ozkan Danis

Ayse Ogan

Pinar Tatlican

Azade Attar

Emrah Cakmakci

Bulent Mertoglu

Meral Birbir

Affiliations

Soon will be listed here.

Abstract

Halophilic archaea offer a potential source for production of polyhydroxyalkanoates (PHAs). Hence, the experiments were carried out with five extremely halophilic archaeal isolates to determine the highest PHA-producing strain. PHA production of each isolates was separately examined in cheap carbon sources such as corn starch, sucrose, whey, apple, melon and tomato wastes. Corn starch was found to be a fairly effective substrate for PHA production. Among the strains studied here, the strain with the highest capability for PHA biosynthesis was found to be 1KYS1. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that 1KYS1 closely related to species of the genus Natrinema. The closest phylogenetic similarity was with the strain of Natrinema pallidum JCM 8980 (99 %). PHA content of 1KYS1 was about 53.14 % of the cell dry weight when starch was used as a carbon source. The formation of large and uniform PHA granules was confirmed by transmission electron microscopy and the biopolymer was identified as poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV). PHBV produced by 1KYS1 was blended with low molar mass polyethylene glycol (PEG 300) to prepare biocompatible films for drug delivery. Rifampicin was used as a model drug and its release from PHBV films was investigated at pH 7.4, 37 °C. It was found that PHBV films obtained from 1KYS1 were very effective for drug delivery. In conclusion, PHBV of 1KYS1 may have a potential usage in drug delivery applications.

Citing Articles

The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles.

Samrot A, Samanvitha S, Shobana N, Renitta E, Senthilkumar P, Kumar S Polymers (Basel). 2021; 13(19).

PMID: 34641118 PMC: 8512352. DOI: 10.3390/polym13193302.

Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic sp. B14-6.

Choi T, Park Y, Song H, Lee S, Park S, Lee H Polymers (Basel). 2021; 13(9).

PMID: 33925903 PMC: 8123457. DOI: 10.3390/polym13091398.

Antibacterial Composite Materials Based on the Combination of Polyhydroxyalkanoates With Selenium and Strontium Co-substituted Hydroxyapatite for Bone Regeneration.

Marcello E, Maqbool M, Nigmatullin R, Cresswell M, Jackson P, Basnett P Front Bioeng Biotechnol. 2021; 9:647007.

PMID: 33898403 PMC: 8059794. DOI: 10.3389/fbioe.2021.647007.

Haloarchaea as Cell Factories to Produce Bioplastics.

Simo-Cabrera L, Garcia-Chumillas S, Hagagy N, Saddiq A, Tag H, Selim S Mar Drugs. 2021; 19(3).

PMID: 33803653 PMC: 8003077. DOI: 10.3390/md19030159.

Production of Poly(3-Hydroxybutyrate) by , , and Haloarchaeal Genera Using Starch as a Carbon Source.

Karray F, Ben Abdallah M, Baccar N, Zaghden H, Sayadi S Archaea. 2021; 2021:8888712.

PMID: 33574733 PMC: 7860971. DOI: 10.1155/2021/8888712.

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