Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Sp. B14-6

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2021 Apr 30

PMID 33925903

Citations 15

Authors

Tae-Rim Choi

Ye-Lim Park

Hun-Suk Song

Sun Mi Lee

Sol Lee Park

Hye Soo Lee

Hyun-Joong Kim

Shashi Kant Bhatia

Ranjit Gurav

Kwon-Young Choi

Yoo Kyung Lee

Yung-Hun Yang

Affiliations

Soon will be listed here.

Abstract

Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (). sp. B14-6 produced poly(6% 3-hydroxybutyrate--94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl--mcl PHA determined a glass transition temperature (T) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 10, weight average molecular weight of 9.1 × 10, and polydispersity index value of 2.5. Overall, the novel sp. B14-6 produced a polymer with high medium-chain-length content, low T, and low crystallinity, indicating its potential use in medical applications.

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