MALDI Imaging Mass Spectrometry: Current Frontiers and Perspectives in Pathology Research and Practice

Overview

Journal Lab Invest

Specialty Pathology

Date 2015 Jan 27

PMID 25621874

Citations 166

Authors

Michaela Aichler

Axel Walch

Affiliations

Soon will be listed here.

Abstract

MALDI Imaging mass spectrometry has entered the field of tissue-based research by providing unique advantages for analyzing tissue specimen in an unprecedented detail. A broad spectrum of analytes ranging from proteins, peptides, protein modification over small molecules, drugs and their metabolites as well as pharmaceutical components, endogenous cell metabolites, lipids, and other analytes are made accessible by this in situ technique in tissue. Some of them were even not accessible in tissues within the histological context before. Thereby, the great advantage of MALDI Imaging is the correlation of molecular information with traditional histology by keeping the spatial localization information of the analytes after mass spectrometric measurement. This method is label-free and allows multiplex analysis of hundreds to thousands of molecules in the very same tissue section simultaneously. Imaging mass spectrometry brings a new quality of molecular data and links the expert discipline of pathology and deep molecular mass spectrometric analysis to tissue-based research. This review will focus on state-of-the-art of MALDI Imaging mass spectrometry, its recent applications by analyzing tissue specimen and the contributions in understanding the biology of disease as well as its perspectives for pathology research and practice.

Citing Articles

Metabolic heterogeneity in tumor cells impacts immunology in lung squamous cell carcinoma.

Wang Q, Sun N, Zhang C, Kunzke T, Zens P, Feuchtinger A Oncoimmunology. 2025; 14(1):2457797.

PMID: 39924768 PMC: 11812363. DOI: 10.1080/2162402X.2025.2457797.

Current state and future prospects of spatial biology in colorectal cancer.

Carranza F, Diaz F, Ninova M, Velazquez-Villarreal E Front Oncol. 2024; 14:1513821.

PMID: 39711954 PMC: 11660798. DOI: 10.3389/fonc.2024.1513821.

Simultaneous noninvasive quantification of redox and downstream glycolytic fluxes reveals compartmentalized brain metabolism.

Patel S, Porcari P, Coffee E, Kim N, Berishaj M, Peyear T Sci Adv. 2024; 10(51):eadr2058.

PMID: 39705365 PMC: 11661454. DOI: 10.1126/sciadv.adr2058.

The incorporation of MALDI mass spectrometry imaging in studies to identify markers of toxicity following opioid exposures in mouse fetuses.

Barnette D, Inselman A, Kaldhone P, Lee G, Davis K, Sarkar S Front Toxicol. 2024; 6:1452974.

PMID: 39691158 PMC: 11651024. DOI: 10.3389/ftox.2024.1452974.

Mass-spectrometry via oscillatory motion in deep pulsed optical lattices.

Gerakis A, Shneider M, Barker P Heliyon. 2024; 10(22):e40410.

PMID: 39679107 PMC: 11639372. DOI: 10.1016/j.heliyon.2024.e40410.

References

Lazova R, Seeley E, Keenan M, Gueorguieva R, Caprioli R . Imaging mass spectrometry--a new and promising method to differentiate Spitz nevi from Spitzoid malignant melanomas. Am J Dermatopathol. 2011; 34(1):82-90. PMC: 3263382. DOI: 10.1097/DAD.0b013e31823df1e2. View

Reyzer M, Hsieh Y, Ng K, Korfmacher W, Caprioli R . Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom. 2003; 38(10):1081-92. DOI: 10.1002/jms.525. View

Norris J, Caprioli R . Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev. 2013; 113(4):2309-42. PMC: 3624074. DOI: 10.1021/cr3004295. View

Hayasaka T, Goto-Inoue N, Zaima N, Kimura Y, Setou M . Organ-specific distributions of lysophosphatidylcholine and triacylglycerol in mouse embryo. Lipids. 2009; 44(9):837-48. DOI: 10.1007/s11745-009-3331-5. View

Meding S, Nitsche U, Balluff B, Elsner M, Rauser S, Schone C . Tumor classification of six common cancer types based on proteomic profiling by MALDI imaging. J Proteome Res. 2012; 11(3):1996-2003. DOI: 10.1021/pr200784p. View

Stoeckli M, Chaurand P, Hallahan D, Caprioli R . Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med. 2001; 7(4):493-6. DOI: 10.1038/86573. View

Pirman D, Reich R, Kiss A, Heeren R, Yost R . Quantitative MALDI tandem mass spectrometric imaging of cocaine from brain tissue with a deuterated internal standard. Anal Chem. 2012; 85(2):1081-9. DOI: 10.1021/ac302960j. View

MKoma A, Seeley E, Washington M, Schwartz D, Muldoon R, Herline A . Proteomic profiling of mucosal and submucosal colonic tissues yields protein signatures that differentiate the inflammatory colitides. Inflamm Bowel Dis. 2010; 17(4):875-83. PMC: 2997147. DOI: 10.1002/ibd.21442. View

Bessede E, Angla-Gre M, Delagarde Y, Sep Hieng S, Menard A, Megraud F . Matrix-assisted laser-desorption/ionization biotyper: experience in the routine of a University hospital. Clin Microbiol Infect. 2010; 17(4):533-8. DOI: 10.1111/j.1469-0691.2010.03274.x. View

10.

Garrett T, Dawson W . Lipid geographical analysis of the primate macula by imaging mass spectrometry. Methods Mol Biol. 2009; 579:247-60. DOI: 10.1007/978-1-60761-322-0_12. View

11.

van Remoortere A, van Zeijl R, van den Oever N, Franck J, Longuespee R, Wisztorski M . MALDI imaging and profiling MS of higher mass proteins from tissue. J Am Soc Mass Spectrom. 2010; 21(11):1922-9. DOI: 10.1016/j.jasms.2010.07.011. View

12.

Hattori K, Kajimura M, Hishiki T, Nakanishi T, Kubo A, Nagahata Y . Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry. Antioxid Redox Signal. 2010; 13(8):1157-67. PMC: 2956403. DOI: 10.1089/ars.2010.3290. View

13.

Rompp A, Guenther S, Takats Z, Spengler B . Mass spectrometry imaging with high resolution in mass and space (HR(2) MSI) for reliable investigation of drug compound distributions on the cellular level. Anal Bioanal Chem. 2011; 401(1):65-73. DOI: 10.1007/s00216-011-4990-7. View

14.

Quaas A, Bahar A, Von Loga K, Seddiqi A, Singer J, Omidi M . MALDI imaging on large-scale tissue microarrays identifies molecular features associated with tumour phenotype in oesophageal cancer. Histopathology. 2013; 63(4):455-62. DOI: 10.1111/his.12193. View

15.

Wu J, Halushka M, Argani P . Intratumoral heterogeneity of HER-2 gene amplification and protein overexpression in breast cancer. Hum Pathol. 2010; 41(6):914-7. PMC: 2958099. DOI: 10.1016/j.humpath.2009.10.022. View

16.

Berman D, Bosenberg M, Orwant R, Thurberg B, Draetta G, Fletcher C . Investigative pathology: leading the post-genomic revolution. Lab Invest. 2011; 92(1):4-8. DOI: 10.1038/labinvest.2011.147. View

17.

Pavlidis N, Pentheroudakis G . Cancer of unknown primary site. Lancet. 2012; 379(9824):1428-35. DOI: 10.1016/S0140-6736(11)61178-1. View

18.

Rujoi M, Estrada R, Yappert M . In situ MALDI-TOF MS regional analysis of neutral phospholipids in lens tissue. Anal Chem. 2004; 76(6):1657-63. DOI: 10.1021/ac0349680. View

19.

Benabdellah F, Yu H, Brunelle A, Laprevote O, de la Porte S . MALDI reveals membrane lipid profile reversion in MDX mice. Neurobiol Dis. 2009; 36(2):252-8. DOI: 10.1016/j.nbd.2009.07.013. View

20.

Schwamborn K, Krieg R, Jirak P, Ott G, Knuchel R, Rosenwald A . Application of MALDI imaging for the diagnosis of classical Hodgkin lymphoma. J Cancer Res Clin Oncol. 2010; 136(11):1651-5. DOI: 10.1007/s00432-010-0823-x. View