Characterization of Rotavirus Subgroup-specific Monoclonal Antibodies and Use in Single-sandwich ELISA Systems for Rapid Subgrouping of Human Strains

Overview

Journal Arch Virol

Specialty Microbiology

Date 1989 Jan 1

PMID 2554860

Citations 2

Authors

G Gerna

A Sarasini

M Torsellini

A di Matteo

F Baldanti

M Parea

M Battaglia

Affiliations

Soon will be listed here.

Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

Citing Articles

Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype.

Gerna G, Sarasini A, Zentilin L, di Matteo A, Miranda P, Parea M Arch Virol. 1990; 112(1-2):27-40.

PMID: 2164375 DOI: 10.1007/BF01348983.

Isolation and characterization of two distinct human rotavirus strains with G6 specificity.

Gerna G, Sarasini A, Parea M, Arista S, Miranda P, Brussow H J Clin Microbiol. 1992; 30(1):9-16.

PMID: 1370851 PMC: 264989. DOI: 10.1128/jcm.30.1.9-16.1992.

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