Expression of Fibroblast Growth Factor by F9 Teratocarcinoma Cells As a Function of Differentiation

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1989 Jun 1

PMID 2544608

Citations 5

Authors

S J Braunhut

L J Gudas

T Kurokawa

J Sasse

P A DAmore

Affiliations

Soon will be listed here.

Abstract

F9 teratocarcinoma stem cells treated with retinoic acid (RA) and dibutyryl cAMP (but2 cAMP) differentiate into embryonic parietal endoderm. Using heparin-affinity chromatography, endothelial cell proliferation assays, immunoprecipitation, and Western analysis with antibodies specific for acidic and basic fibroblast growth factors (FGFs), we detected biologically active FGF in F9 cells only after differentiation. A bovine basic FGF cDNA probe hybridized to 2.2-kb mRNAs in both F9 stem and parietal endoderm cells and to a 3.8-kb mRNA in F9 stem cells. A genomic DNA probe for acidic FGF hybridized to a 5.8-6.0-kb mRNA in both F9 stem and parietal endoderm cells, and to a 6.0-6.3-kb mRNA only in parietal endoderm cells. Although these FGF mRNAs were present in the stem cells, we could find no evidence that F9 stem cells synthesized FGFs, whereas differentiated F9 cells synthesized both acidic and basic FGF-like proteins. We conclude that biologically active factors with properties characteristic of acidic and basic FGF are expressed by F9 parietal endoderm cells after differentiation. Differentiating embryonic parietal endoderm thus may serve as a source of FGF molecules in the developing blastocyst, where these factors appear to play a central role in subsequent embryogenesis.

Citing Articles

Heparin-binding growth factors and their receptors.

Olwin B Cytotechnology. 2012; 2(4):351-65.

PMID: 22358874 DOI: 10.1007/BF00364998.

The meninges is a source of retinoic acid for the late-developing hindbrain.

Zhang J, Smith D, Yamamoto M, Ma L, McCaffery P J Neurosci. 2003; 23(20):7610-20.

PMID: 12930800 PMC: 6740759.

Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells.

Tiesman J, Rizzino A In Vitro Cell Dev Biol. 1989; 25(12):1193-8.

PMID: 2481673 DOI: 10.1007/BF02621274.

Secretion of polypeptides related to epidermal growth factor and insulinlike growth factor I by a human teratocarcinoma cell line.

Jing N, Shiurba R, Kitani H, Kawakatsu H, Tomooka Y, Sakakura T In Vitro Cell Dev Biol. 1991; 27A(11):864-72.

PMID: 1660868 DOI: 10.1007/BF02630989.

Expression of fibroblast growth factor receptors by embryonal carcinoma cells and early mouse embryos.

Campbell W, Miller K, Anderson T, Shull J, Rizzino A In Vitro Cell Dev Biol. 1992; 28A(1):61-6.

PMID: 1370443 DOI: 10.1007/BF02631080.

References

Wang S, LaRosa G, Gudas L . Molecular cloning of gene sequences transcriptionally regulated by retinoic acid and dibutyryl cyclic AMP in cultured mouse teratocarcinoma cells. Dev Biol. 1985; 107(1):75-86. DOI: 10.1016/0012-1606(85)90377-x. View

Lawrence D, Pircher R, Jullien P . Normal embryo fibroblasts release transforming growth factors in a latent form. J Cell Physiol. 1984; 121(1):184-8. DOI: 10.1002/jcp.1041210123. View

Togari A, Dickens G, Kuzuya H, Guroff G . The effect of fibroblast growth factor on PC12 cells. J Neurosci. 1985; 5(2):307-16. PMC: 6565190. View

Klagsbrun M, Shing Y . Heparin affinity of anionic and cationic capillary endothelial cell growth factors: analysis of hypothalamus-derived growth factors and fibroblast growth factors. Proc Natl Acad Sci U S A. 1985; 82(3):805-9. PMC: 397135. DOI: 10.1073/pnas.82.3.805. View

Vasseur M, Duprey P, Brulet P, Jacob F . One gene and one pseudogene for the cytokeratin endo A. Proc Natl Acad Sci U S A. 1985; 82(4):1155-9. PMC: 397213. DOI: 10.1073/pnas.82.4.1155. View

Yoshida T, Miyagawa K, Odagiri H, Sakamoto H, LITTLE P, Terada M . Genomic sequence of hst, a transforming gene encoding a protein homologous to fibroblast growth factors and the int-2-encoded protein. Proc Natl Acad Sci U S A. 1987; 84(20):7305-9. PMC: 299281. DOI: 10.1073/pnas.84.20.7305. View

Weeks D, Melton D . A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta. Cell. 1987; 51(5):861-7. DOI: 10.1016/0092-8674(87)90109-7. View

Kimelman D, Kirschner M . Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell. 1987; 51(5):869-77. DOI: 10.1016/0092-8674(87)90110-3. View

Rizzino A, Kuszynski C, Ruff E, Tiesman J . Production and utilization of growth factors related to fibroblast growth factor by embryonal carcinoma cells and their differentiated cells. Dev Biol. 1988; 129(1):61-71. DOI: 10.1016/0012-1606(88)90161-3. View

10.

Sternfeld M, Hendrickson J, Keeble W, Rosenbaum J, Robertson J, Pittelkow M . Differential expression of mRNA coding for heparin-binding growth factor type 2 in human cells. J Cell Physiol. 1988; 136(2):297-304. DOI: 10.1002/jcp.1041360212. View

11.

Shimasaki S, Emoto N, Koba A, Mercado M, Shibata F, Cooksey K . Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA. Biochem Biophys Res Commun. 1988; 157(1):256-63. DOI: 10.1016/s0006-291x(88)80041-x. View

12.

Yoshida T, Muramatsu H, Muramatsu T, Sakamoto H, Katoh O, Sugimura T . Differential expression of two homologous and clustered oncogenes, Hst1 and Int-2, during differentiation of F9 cells. Biochem Biophys Res Commun. 1988; 157(2):618-25. DOI: 10.1016/s0006-291x(88)80295-x. View

13.

Folkman J, LONG Jr D, Becker F . Growth and metastasis of tumor in organ culture. Cancer. 1963; 16:453-67. DOI: 10.1002/1097-0142(196304)16:4<453::aid-cncr2820160407>3.0.co;2-y. View

14.

GOSPODAROWICZ D, Cheng J, Lui G, Fujii D, Baird A, Bohlen P . Fibroblast growth factor in the human placenta. Biochem Biophys Res Commun. 1985; 128(2):554-62. DOI: 10.1016/0006-291x(85)90082-8. View

15.

Rizzino A, Bowen-Pope D . Production of PDGF-like growth factors by embryonal carcinoma cells and binding of PDGF to their endoderm-like differentiated cells. Dev Biol. 1985; 110(1):15-22. DOI: 10.1016/0012-1606(85)90058-2. View

16.

Burgess W, Mehlman T, Friesel R, JOHNSON W, Maciag T . Multiple forms of endothelial cell growth factor. Rapid isolation and biological and chemical characterization. J Biol Chem. 1985; 260(21):11389-92. View

17.

Esch F, Baird A, Ling N, Ueno N, Hill F, Denoroy L . Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci U S A. 1985; 82(19):6507-11. PMC: 390746. DOI: 10.1073/pnas.82.19.6507. View

18.

Dony C, Kessel M, Gruss P . Post-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9. Nature. 1985; 317(6038):636-9. DOI: 10.1038/317636a0. View

19.

GOSPODAROWICZ D, Cheng J, Lui G, Baird A, Esch F, Bohlen P . Corpus luteum angiogenic factor is related to fibroblast growth factor. Endocrinology. 1985; 117(6):2383-91. DOI: 10.1210/endo-117-6-2383. View

20.

Gimenez-Gallego G, Rodkey J, Bennett C, DiSalvo J, Thomas K . Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies. Science. 1985; 230(4732):1385-8. DOI: 10.1126/science.4071057. View