MiR-219-5p Modulates Cell Growth of Papillary Thyroid Carcinoma by Targeting Estrogen Receptor α

Overview

Journal J Clin Endocrinol Metab

Publisher Oxford University Press

Specialty Endocrinology

Date 2014 Nov 26

PMID 25423566

Citations 32

Authors

Chen Huang

Zhaogeng Cai

Mingzhu Huang

Chaoming Mao

Qifa Zhang

Yi Lin

Xiaomei Zhang

Bi Tang

Yuqing Chen

Xiaojing Wang

Zhongqing Qian

Lei Ye

Yongde Peng

Huanbai Xu

Affiliations

Soon will be listed here.

Abstract

Context: Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. It has been demonstrated that micro-RNAs (miRNAs) are involved in the development of PTC. The miRNA-chromatin immunoprecipitation microarray assay revealed down-regulation of miR-219-5p; however, the effect of miR-219-5p on PTC cell growth remains unknown. This result implied the critical role of miR-219-5p in the development of PTC.

Methods: We investigated the association between miR-219-5p and PTC development. Expression of miR-219-5p was monitored in 30 PTC tissue specimens and compared with that in 30 normal thyroid tissue specimens. The effect of miR-219-5p on PTC development was studied by cell proliferation, migration, and apoptosis assays. The underlying mechanism was clarified by a reporter assay and rescue experiment.

Results: The current study confirmed that miR-219-5p expression was inhibited in PTC tissue samples. There were statistically significant differences in the expression of miR-219-5p with regard to sex, tumor size, and lymph node metastasis in patients with PTC. Forced expression of miR-219-5p suppressed PTC cell proliferation and migration and promoted apoptosis. Further study showed that estrogen receptor (ER) α was the direct target of miR-219-5p and mediated the effect of miR-219-5p on PTC occurrence. Expression of miR-219-5p was inversely correlated with that of ERα. Importantly, ERα overexpression in PTC cells rescued the inhibitory effect of miR-219-5p on PTC cell proliferation and migration. Thus, our results indicated that miR-219-5p played a critical role in PTC growth by inhibiting ERα.

Conclusion: Our investigation identified miR-219-5p as a negative regulator of PTC development through targeting of ERα.

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